Prevalence, genetic relatedness and antibiotic resistance of hospital-acquired clostridium difficile PCR ribotype 018 strains
| Autor: | Dong Gyun Lim, Mi Ran Seo, Hyunjoo Pai, Yangsoon Lee, Jieun Kim |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Microbial Sensitivity Tests Biology Multiple Loci VNTR Analysis Polymerase Chain Reaction Ribotyping Tazobactam Microbiology Tertiary Care Centers 03 medical and health sciences 0302 clinical medicine Antibiotic resistance Drug Resistance Bacterial Republic of Korea Prevalence medicine Humans Pharmacology (medical) 030212 general & internal medicine Cross Infection Clostridioides difficile Clindamycin General Medicine Clostridium difficile Anti-Bacterial Agents Metronidazole Infectious Diseases Clostridium Infections Vancomycin Multilocus Sequence Typing Piperacillin medicine.drug |
| Zdroj: | International Journal of Antimicrobial Agents. 51:762-767 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2018.01.025 |
| Popis: | Clostridium difficile infection (CDI) is a major healthcare-associated infection. The aim of this study was to investigate the genetic relatedness of the endemic C. difficile PCR ribotype 018 strains in an institution and changes to their characteristics during a five-year period. A total of 207 isolates from inpatients at Hanyang University Hospital from 2009 to 2013 were analysed using multilocus variable-number tandem-repeat analysis (MLVA). Minimum inhibitory concentrations (MICs) of several antibiotics were determined. In total, 204 (98.6%) were genetically related, with a summed tandem-repeat distance (STRD) ≤ 10. Minimum-spanning-tree analysis identified 78 MLVA types, categorized into six clonal complexes (CCs). The largest cluster, CC-I, included 51 MLVA types from 148 isolates (71.5%) and the second largest cluster, CC-II, included 10 MLVA types from 36 isolates (17.4%). Resistance rates for antibiotics were: clindamycin (CLI), 97.6%; moxifloxacin (MXF), 98.6%; vancomycin (VAN), 1.4%; and rifaximin (RFX), 8.2%. All isolates were susceptible to piperacillin/tazobactam (TZP) and metronidazole (MTZ). Comparing the MICs of antibiotics for the isolates each year from 2009 to 2013, MICs of antibiotics that promote CDI, such as CLI, MXF, TZP and RFX, increased over the five-year period (P-value by Kruskal-Wallis test |
| Databáze: | OpenAIRE |
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