Remodeling of the Purkinje Network in Congestive Heart Failure in the Rabbit
| Autor: | Sunil Jit R. J. Logantha, Joseph Yanni, Ashraf Kitmitto, Oliver J. Monfredi, Luke Stuart, Akbar Vohra, Shu Nakao, R Hutcheon, Tobias Starborg, Caroline Jones, GM Quigley, George Hart, Robert S. Stephenson, Jonathan C. Jarvis, X. Cai, Antonio F. Corno, Il Young Oh, Halina Dobrzynski, Mark R. Boyett |
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| Rok vydání: | 2021 |
| Předmět: |
Male
rabbits Action Potentials Purkinje fibers 030204 cardiovascular system & hematology RC1200 Electrocardiography 0302 clinical medicine Heart Rate Tomography 0303 health sciences Ventricular Remodeling Cardiac Pacing Artificial ion channels Rabbit (nuclear engineering) medicine.anatomical_structure Ion channels Models Animal ComputingMethodologies_DOCUMENTANDTEXTPROCESSING Cardiology Rabbits Cardiology and Cardiovascular Medicine RM medicine.medical_specialty Heart Ventricles Heart failure tomography 03 medical and health sciences Internal medicine Electron microscopy medicine Animals Ventricular conduction Ion channel 030304 developmental biology Heart Failure electron microscopy business.industry Original Articles X-Ray Microtomography medicine.disease R1 business |
| Zdroj: | Circulation. Heart Failure Logantha, S J R J, Cai, X J, Yanni, J, Jones, C B, Stephenson, R S, Stuart, L, Quigley, G, Monfredi, O, Nakao, S, Oh, I Y, Starborg, T, Kitmitto, A, Vohra, A, Hutcheon, R C, Corno, A F, Jarvis, J C, Dobrzynski, H, Boyett, M R & Hart, G 2021, ' Remodeling of the Purkinje Network in Congestive Heart Failure in the Rabbit ', Circulation. Heart Failure, vol. 14, no. 7, pp. 800-816 . https://doi.org/10.1161/CIRCHEARTFAILURE.120.007505 |
| ISSN: | 1941-3297 1941-3289 |
| DOI: | 10.1161/circheartfailure.120.007505 |
| Popis: | Supplemental Digital Content is available in the text. Background: Purkinje fibers (PFs) control timing of ventricular conduction and play a key role in arrhythmogenesis in heart failure (HF) patients. We investigated the effects of HF on PFs. Methods: Echocardiography, electrocardiography, micro-computed tomography, quantitative polymerase chain reaction, immunohistochemistry, volume electron microscopy, and sharp microelectrode electrophysiology were used. Results: Congestive HF was induced in rabbits by left ventricular volume- and pressure-overload producing left ventricular hypertrophy, diminished fractional shortening and ejection fraction, and increased left ventricular dimensions. HF baseline QRS and corrected QT interval were prolonged by 17% and 21% (mean±SEMs: 303±6 ms HF, 249±11 ms control; n=8/7; P=0.0002), suggesting PF dysfunction and impaired ventricular repolarization. Micro-computed tomography imaging showed increased free-running left PF network volume and length in HF. mRNA levels for 40 ion channels, Ca2+-handling proteins, connexins, and proinflammatory and fibrosis markers were assessed: 50% and 35% were dysregulated in left and right PFs respectively, whereas only 12.5% and 7.5% changed in left and right ventricular muscle. Funny channels, Ca2+-channels, and K+-channels were significantly reduced in left PFs. Microelectrode recordings from left PFs revealed more negative resting membrane potential, reduced action potential upstroke velocity, prolonged duration (action potential duration at 90% repolarization: 378±24 ms HF, 249±5 ms control; n=23/38; P |
| Databáze: | OpenAIRE |
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