High beta-HPV DNA loads and strong seroreactivity are present in epidermodysplasia verruciformis
| Autor: | Kristina M. Michael, Herbert Pfister, Ada Amantea, Michele Mondini, Marco De Andrea, Santo Landolfo, Giorgio Leigheb, Marisa Gariglio, Tim Waterboer, Sönke Weissenborn, Michael Pawlita, Barbara Azzimonti, Valentina Dell'Oste, Elisa Zavattaro |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Concordance Eyebrow Dermatology Biology Antibodies Viral Biochemistry Virus eyebrow hairs Genotype medicine Betapapillomavirus Humans human papillomavirus Molecular Biology In Situ Hybridization integumentary system epidermodysplasia verruciformis non-melanoma kin cancer virus diseases Cell Biology Epidermodysplasia verruciformis Middle Aged Viral Load medicine.disease Titer medicine.anatomical_structure DNA Viral Epidermodysplasia Verruciformis biology.protein Antibody Viral load |
| Zdroj: | The Journal of investigative dermatology. 129(4) |
| ISSN: | 1523-1747 |
| Popis: | Epidermodysplasia verruciformis (EV) is a rare disease, characterized by cutaneous warts and associated with a strong predisposition to beta-genus human papillomavirus (HPV). Earlier studies reported high copy numbers of HPV-DNA in nearly all skin tumors from EV patients, but neither HPV replication status in non-lesional skin nor anti-HPV seroreactivity in these patients have been reported yet. We therefore performed a comprehensive viral load analysis for the more common beta-HPV types on skin samples and plucked eyebrow hairs from four EV patients treated at our dermatology department. The results clearly demonstrate that they carry a multiplicity (up to eighteen types) of beta-HPV genotypes in both skin sites. Worthy of note, a high intrapatient concordance for specific types between hair bulbs and skin biopsies was observed and the same beta-PV profile was maintained over time. Viral load analysis revealed a load range between less than one HPV-DNA copy per 100 cells to more than 400 HPV-DNA copies per cell in both eyebrow hairs and skin proliferative lesions. Evaluation of seroreactivity to beta-HPV types in the four EV patients revealed that antibodies against the 16 beta-HPV were significantly more prevalent and showed higher titers than in the controls. |
| Databáze: | OpenAIRE |
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