Regional changes in cardiac and stellate ganglion norepinephrine transporter in DOCA–salt hypertension
| Autor: | Beth A. Habecker, Lindsay M. Parker, Mohammad Esfahanian, Martin Novotny, Greg M. Swain, John M. Spitsbergen, Erica A. Wehrwein, David L. Kreulen |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Stellate Ganglion Enzyme-Linked Immunosorbent Assay Real-Time Polymerase Chain Reaction Doca salt Article Protein expression Reuptake Rats Sprague-Dawley Norepinephrine (medication) Desoxycorticosterone Acetate Cellular and Molecular Neuroscience Mineralocorticoids Internal medicine medicine Animals Sodium Chloride Dietary Messenger RNA Norepinephrine Plasma Membrane Transport Proteins biology Endocrine and Autonomic Systems Myocardium Heart Immunohistochemistry Rats Disease Models Animal Endocrinology medicine.anatomical_structure Norepinephrine transporter Ventricle Stellate ganglion Hypertension biology.protein Neurology (clinical) medicine.drug |
| Zdroj: | Autonomic Neuroscience. 179:99-107 |
| ISSN: | 1566-0702 |
| DOI: | 10.1016/j.autneu.2013.08.070 |
| Popis: | Uptake of norepinephrine via the neuronal norepinephrine transporter is reduced in the heart during deoxycorticosterone (DOCA)-salt hypertension. We hypothesized that this was due to reduced norepinephrine transporter mRNA and/or protein expression in the stellate ganglia and heart. After 4-weeks of DOCA-salt treatment there was no change in norepinephrine transporter mRNA in either the right or the left stellate ganglia from hypertensive rats (n=5–7, p>0.05). Norepinephrine transporter immunoreactivity in the left stellate ganglion was significantly increased (n=4, p0.05). Whole heart norepinephrine content was significantly reduced in DOCA rats consistent with reduced uptake function; however, when norepinephrine was assessed by chamber, a significant decrease was noted only in the right atrium and right ventricle (n=6, p0.05). Therefore, 1) contrary to our hypothesis reduced reuptake in the hypertensive heart is not exclusively due to an overall reduction in norepinephrine transporter mRNA or protein in the stellate ganglion or heart, and 2) norepinephrine transporter regulation occurs regionally in the heart and stellate ganglion in the hypertensive rat heart. |
| Databáze: | OpenAIRE |
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