Cloning, expression and stress-respondent transcription of long-chain acyl-coenzyme A synthetase cDNA gene of Nannochloropsis gaditana and its involvement in the biosynthesis of eicosapentaenoic and decosahexaenoic acids
| Autor: | Yi-jiang Huang, Ming-gang Zheng, Li Zheng, Zhong-tao Sun, Ling Wang |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Complementary Docosahexaenoic Acids biology Saccharomyces cerevisiae Bioengineering General Medicine biology.organism_classification Applied Microbiology and Biotechnology Eicosapentaenoic acid Recombinant Proteins chemistry.chemical_compound Isoelectric point Eicosapentaenoic Acid Biochemistry Biosynthesis chemistry Stress Physiological Docosahexaenoic acid Complementary DNA Coenzyme A Ligases Gene Peptide sequence Stramenopiles Biotechnology |
| Zdroj: | Biotechnology Letters. 36:141-145 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-013-1342-z |
| Popis: | A novel cDNA gene, NgLACS, that encodes a long-chain acyl-CoA sythetase (LACS), was cloned from Nannochloropsis gaditana and characterized. The cDNA was 2,360 bp in length, consisting of an ORF of 1,950 bp, a 5'-untranslated region of 88 bp and a 3'-untranslated region of 322 bp. The deduced amino acid sequence of LACS was 649 amino acid residues in length with a predicted molecular weight of 71 kDa and an isoelectric point of pH 7.8. When the alga was treated with excessive nitrogen and iron, and at 15 °C, the proportion of long-chain polyunsaturated acyl-CoAs in the total acyl-CoAs and the abundance of NgLACS cDNA gene transcript were up-regulated. Over-expression of NgLACS in Saccharomyces cerevisiae caused the accumulation of eicosapentaenoic acid and docosahexaenoic acid. |
| Databáze: | OpenAIRE |
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