Effects of steam autoclave treatment on Geobacillus stearothermophilus spores
| Autor: | Shiwei Wang, George Korza, Milomir Suvira, Kayla Rosenbeck, Luz del Carmen Huesca-Espitia, Peter Setlow, William Li, Yong-qing Li, Barbara Setlow |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030106 microbiology Biology Applied Microbiology and Biotechnology Endospore Microbiology Autoclave Geobacillus stearothermophilus 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Picolinic Acids Spores Bacterial fungi Sterilization General Medicine Sterilization (microbiology) Dipicolinic acid Spore Steam 030104 developmental biology chemistry Germination Mutation Bacterial spore Biotechnology |
| Zdroj: | Journal of applied microbiology. 121(5) |
| ISSN: | 1365-2672 |
| Popis: | Aims To determine the mechanism of autoclave killing of Geobacillus stearothermophilus spores used in biological indicators (BIs) for steam autoclave sterilization, and rates of loss of spore viability and a spore enzyme used in BIs. Methods and Results Spore viability, dipicolinic acid release, nucleic acid staining, α-glucosidase activity, protein structure and mutagenesis were measured during autoclaving of G. stearothermophilus spores. Loss of dipicolinic acid and increases in spore core nucleic acid staining were slower than loss of spore viability. Spore core α-glucosidase was also lost more slowly than spore viability, although soluble α-glucosidase in spore preparations was lost more rapidly. However, spores exposed to an effective autoclave sterilization lost all viability and α-glucosidase activity. Apparently killed autoclaved spores were not recovered by artificial germination in supportive media, much spore protein was denatured during autoclaving, and partially killed autoclave-treated spore preparations did not acquire mutations. Conclusions These results indicate that autoclave-killed spores cannot be revived, spore killing by autoclaving is likely by protein damage, and spore core α-glucosidase activity is lost more slowly than spore viability. Significance and Impact of Study This work provides insight into the mechanism of autoclave killing of spores of an organism used in BIs, and that a spore enzyme in a BI is more stable to autoclaving than spore viability. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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