Platelet storage in Fresenius/NPBI polyolefin and BTHC-PVC bags: a direct comparison
| Autor: | Olive Drummond, K. McColl, Christopher Prowse, Ian MacGregor, V. Hornsey |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Blood Platelets
Time Factors Chromatography Lysis Polyenes Hematology Buffy coat Carbon Dioxide Polyolefin Oxygen Butyrates Polyvinyl chloride chemistry.chemical_compound Hypotonic Shock chemistry Blood Preservation In vivo Immunology Product Packaging Humans Platelet Mean platelet volume Polyvinyl Chloride |
| Zdroj: | Transfusion Medicine. 18:223-227 |
| ISSN: | 1365-3148 0958-7578 |
| DOI: | 10.1111/j.1365-3148.2008.00869.x |
| Popis: | New platelet storage systems, such as changes in the plastic of the storage bags, require validation. In this study, pooled buffy coat platelets stored in Fresenius/NPBI polyolefin bags were compared with those stored in Fresenius/NPBI butyryl-trihexyl citrate (BTHC) plasticized polyvinyl chloride (PVC). The CompoSelect thrombocyte polishing filter system (1000 mL polyolefin bag) and the CompoStop F730 system (1300 mL BTHC-PVC bag) were used to prepare paired, plasma-suspended, buffy coat platelet concentrates. Samples were taken up to day 7 for in vitro analysis. In a separate experiment, 12 units were prepared using the CompoStop F730 system and samples taken after leucofiltration for FXIIa assay. By day 7, platelet concentrates stored in BTHC-PVC demonstrated significantly higher pH levels (7.32 +/- 0.05 vs. 7.26 +/- 0.05) and a greater degree of cell lysis as shown by increased lactate dehydrogenase levels (497 +/- 107 vs. 392 +/- 81 U L(-1)). The supernatants contained higher concentrations of soluble P-selectin and the chemokine 'regulated on activation, normal T-cell expressed and presumably secreted', which are released from the alpha-granules during activation. The ATP concentrations were significantly lower in BTHC-PVC. Platelet counts, mean platelet volume and hypotonic shock response were similar for both bags. FXIIa antigen concentrations were 0.6 +/- 0.2 ng mL(-1) indicating that activation of the contact factor pathway had not occurred. Although the CompoStop F730 leucoreduction filter did not activate the contact system, platelets stored in 100% plasma in BTHC-PVC bags demonstrated different in vitro characteristics from those stored in polyolefin. Further work is required to demonstrate whether these differences will affect in vivo recovery and survival. |
| Databáze: | OpenAIRE |
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