Properties of receptor-controlled inositol trisphosphate formation in parotid acinar cells
| Autor: | D L Aub, J W Putney |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
medicine.medical_specialty Inositol Phosphates chemistry.chemical_element Inositol 1 4 5-Trisphosphate In Vitro Techniques Calcium Biochemistry chemistry.chemical_compound Internal medicine Muscarinic acetylcholine receptor medicine Animals Methacholine Compounds Parotid Gland Inositol Receptor Molecular Biology Methacholine Chloride biology Rats Inbred Strains Inositol trisphosphate Cell Biology Inositol trisphosphate receptor Receptors Muscarinic Rats Endocrinology chemistry Gq alpha subunit Second messenger system biology.protein Biophysics Sugar Phosphates Research Article |
| Zdroj: | Biochemical Journal. 225:263-266 |
| ISSN: | 1470-8728 0264-6021 |
| Popis: | Activation of muscarinic receptors in rat parotid cells results in breakdown of polyphosphoinositides liberating inositol phosphates, including inositol trisphosphate. Formation of inositol trisphosphate appears independent of agonist-induced Ca2+ mobilization, since neither formation nor degradation of inositol trisphosphate are appreciably altered in low-calcium media, and elevation of cytosolic Ca2+ with a calcium ionophore does not cause an increase in cellular inositol trisphosphate. Further, activation of substance P receptors and alpha 1-adrenoreceptors, but not beta-adrenoreceptors, increases inositol trisphosphate formation. The dose-response curve for methacholine activation of inositol trisphosphate formation more closely approximates the curve for receptor occupancy than for Ca2+-activated K+ release. These results are all consistent with the suggestion that inositol trisphosphate could function as a second messenger linking receptor occupation to cellular Ca2+ mobilization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|