Quality control of immunocytochemical staining of effusions using a standardized method of cell processing
| Autor: | E. M. C. A. De Bruijn, S C Henzen-Logmans, V Kuenen-Boumeester, P van Loenen |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Mesothelioma Quality Control Pathology medicine.medical_specialty Histology Erythrocytes medicine.drug_class Breast Neoplasms Adenocarcinoma Monoclonal antibody Cell morphology Hemolysis Pathology and Forensic Medicine chemistry.chemical_compound medicine Ascitic Fluid Humans Paraformaldehyde Gastrointestinal Neoplasms Ovarian Neoplasms business.industry Antibodies Monoclonal Prostatic Neoplasms General Medicine medicine.disease Immunohistochemistry Kidney Neoplasms Carcinoembryonic Antigen Endometrial Neoplasms Pleural Effusion chemistry Cytopathology Monoclonal Alkaline phosphatase Female business Immunostaining |
| Zdroj: | Acta cytologica. 40(3) |
| ISSN: | 0001-5547 |
| Popis: | Objective To improve the quality and reproducibility of immunocytochemical staining of effusions by using a standardized method of cell processing. Study design The study included the specimens of 108 effusions (44 benign, 56 adenocarcinoma metastases and 8 mesotheliomas). Hemorrhagic effusions were lysed using isotonic ammonium chloride. All pellets were fixed in 1% paraformaldehyde dissolved in phosphate-buffered saline (PBS), pH 7.4, and washed in PBS. A Burker counting chamber was used to adjust the pellets to a standard cell concentration. The panel of monoclonal antibodies (MAbs) included MOC-31, Ber-EP4 and anticarcinoembryonic antigen (anti-CEA). The alkaline phosphatase/anti-alkaline phosphatase technique was applied. Results Standardized material processing resulted in reproducible specimens with good preservation of cell morphology, reduction of nonspecific interaction and good immunostain intensity. MAbs MOC-31 and Ber-EP4 gave similar results: both were positive in all adenocarcinomas. Anti-CEA was positive in 73%. Benign effusions showed no expression. In contrast to the literature, seven mesotheliomas showed variable membranous expression of MOC-31 and Ber-EP4. Conclusion High-quality immunostaining results were obtained by using a standardized method of cell processing. MAbs MOC-31 and Ber-EP4 cannot be used as differentiation markers between mesotheliomas and adenocarcinomas. Discrepancies in immunocytochemical staining results may be caused partly by differences in cell preparation. |
| Databáze: | OpenAIRE |
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