Hypoxic stress underlies defects in erythroblast islands in the Rb-null mouse
| Autor: | Kay F. Macleod, Benjamin T. Spike, Benjamin C. Dibling |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Programmed cell death
Erythroblasts Liver cytology Immunology Enucleation Red Cells Biochemistry Retinoblastoma Protein Mice Erythroblast In vivo hemic and lymphatic diseases medicine Animals Erythropoiesis Hypoxia Cells Cultured Oligonucleotide Array Sequence Analysis Cell Nucleus Mice Knockout biology Cell Death Integrases Gene Expression Profiling Macrophages Retinoblastoma protein Cell Biology Hematology Cell biology Cell nucleus medicine.anatomical_structure Liver biology.protein |
| Popis: | Definitive erythropoiesis occurs in islands composed of a central macrophage in contact with differentiating erythroblasts. Erythroid maturation including enucleation can also occur in the absence of macrophages both in vivo and in vitro. We reported previously that loss of Rb induces cell-autonomous defects in red cell maturation under stress conditions, while other reports have suggested that the failure of Rb-null erythroblasts to enucleate is due to defects in associated macrophages. Here we show that erythropoietic islands are disrupted by hypoxic stress, such as occurs in the Rb-null fetal liver, that Rb−/− macrophages are competent for erythropoietic island formation in the absence of exogenous stress and that enucleation defects persist in Rb-null erythroblasts irrespective of macrophage function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|