Membrane depolarization inhibits thrombin-induced calcium influx and aggregation in human platelets
| Autor: | Balázs Sarkadi, Attila Tordai, Tünde Kovács, G. Gárdos, Ilma Szász |
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| Rok vydání: | 1990 |
| Předmět: |
Blood Platelets
Cytoplasm Platelet Aggregation Biophysics chemistry.chemical_element Calcium In Vitro Techniques Biochemistry Calcium in biology Membrane Potentials chemistry.chemical_compound Aggregation Receptor-operated calcium channel Structural Biology Calcium flux Genetics Humans Molecular Biology Egtazic Acid Membrane potential Voltage-dependent calcium channel Gramicidin Thrombin Depolarization Cell Biology Calcium ATPase chemistry Human platelet Signal Transduction |
| Zdroj: | FEBS letters. 266(1-2) |
| ISSN: | 0014-5793 |
| Popis: | The relationship between thrombin-evoked changes in intracellular calcium concentration ([Ca2+]i) and aggregation was examined in Indo-1-loaded human platelets. The stimulus-induced intracellular calcium release and external calcium influx, as well as platelet aggregation, were studied in the same cell preparation. A close correlation between the sustained high [Ca2+]i level, depending on calcium entry, and the aggregation response was found. Gramicidin, at a concentration high enough to induce membrane depolarization, strongly inhibited the calcium influx and aggregation, but did not influence the thrombin-induced intracellular calcium release. We conclude that calcium influx through depolarization-inhibited calcium channels is a prerequisite of thrombin-induced platelet aggregation. |
| Databáze: | OpenAIRE |
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