Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy
| Autor: | Valíria Duarte Cerqueira, Aline Amaral Imbeloni, Milene Silveira Ferreira, Pedro Fernando da Costa Vasconcelos, Carlos Alberto de Oliveira Junior, Gabriela Riet Correa Rivero, Robert B. Tesh, Wellington Bandeira da Silva, Franko de Arruda e Silva, Gilmara Abreu da Silva, Juarez Antônio Simões Quaresma, Ana Paula Sousa Araújo, Pedro Soares Bezerra Júnior, Paulo Henrique Gomes de Castro, Jorge Rodrigues de Sousa |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Pathology medicine.medical_specialty Necrosis animal diseases viruses 030231 tropical medicine RC955-962 Spleen Microbiology Virus yellow fever 03 medical and health sciences 0302 clinical medicine Febre Amarela / veterin?ria Arctic medicine. Tropical medicine parasitic diseases medicine Animals Lymph node Saimiri pathophysiology biology business.industry Squirrel monkey Yellow fever virus diseases biology.organism_classification medicine.disease Yellow Fever Virus Infection QR1-502 Disease Models Animal medicine.anatomical_structure experimental infection Saimiri / anatomia & histologia Original Article medicine.symptom V?rus da Febre Amarela / patogenicidade Yellow fever virus business Pyknosis |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 115 (2020) Memórias do Instituto Oswaldo Cruz Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| ISSN: | 1678-8060 |
| Popis: | Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade Federal do Par?. Programa de P?s-Gradua??o em Biologia de Agentes Infecciosos e Parasit?rios. Bel?m, PA, Brasil. Universidade Federal do Par?. Instituto de Medicina Veterin?ria. Laborat?rio de Patologia Animal. Castanhal, PA, Brasil. Universidade Federal do Par?. Instituto de Medicina Veterin?ria. Laborat?rio de Patologia Animal. Castanhal, PA, Brasil. Universidade Federal do Par?. Instituto de Medicina Veterin?ria. Laborat?rio de Patologia Animal. Castanhal, PA, Brasil. Universidade Federal do Par?. Instituto de Medicina Veterin?ria. Laborat?rio de Patologia Animal. Castanhal, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Par?. Departamento de Patologia. Bel?m, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Par?. Departamento de Patologia. Bel?m, PA, Brasil. BACKGROUND Non-human primates contribute to the spread of the yellow fever virus (YFV) and the establishment of transmission cycles in endemic areas. OBJECTIVE To describe the severe histopathological aspects of YFV infection, 10 squirrel monkeys were infected with YFV and blood, brain, liver, kidney, spleen, heart, lung, lymph node and stomach were collected at 1-7, 10, 20 and 30 days post-infection (dpi). METHODS Histopathological analysis and detection of the genome and viral antigens and neutralising antibodies were performed by RT-PCR, immunohistochemistry and neutralisation test, respectively. FINDINGS Only one animal died from the experimental infection. The genome and viral antigens were detected in all investigated organs (1-30 dpi) and the neutralising antibodies from seven to 30 dpi. The brain contained perivascular haemorrhage (6 dpi); in the liver, midzonal haemorrhage and lytic necrosis (6 dpi) were observed. The kidney had bleeding in the Bowman?s capsule and tubular necrosis (6 dpi). Pyknotic lymphocytes were observed in the spleen (1-20 dpi), the lung had haemorrhage (2-6 dpi), in the endocardium it contained nuclear pyknosis and necrosis (2-3 dpi) and the stomach contained blood in the lumen (6 dpi). MAIN FINDINGS Squirrel monkeys reliably reproduced the responses observed in human cases of yellow fever and, therefore, constitute an excellent experimental model for studies on the pathophysiology of the disease. |
| Databáze: | OpenAIRE |
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