Anti-cell proliferative and anti-angiogenic potential of andrographolide during 7,12- dimethylbenz(a)anthracene induced hamster buccal pouch carcinogenesis

Autor: Arjun Kumar Singh, Shanmugam Manoharan, Duraisamy Rajasekaran, K Vasudevan, Asokan Manimaran, K.G. Suresh
Rok vydání: 2013
Předmět:
Male
Vascular Endothelial Growth Factor A
Cancer Research
Epidemiology
Andrographolide
Cell
DMBA
Angiogenesis Inhibitors
Apoptosis
Neovascularization
Immunoenzyme Techniques
chemistry.chemical_compound
Cricetinae
Tumor Cells
Cultured
Cyclin D1
biology
Neovascularization
Pathologic
Reverse Transcriptase Polymerase Chain Reaction
medicine.anatomical_structure
Cell Transformation
Neoplastic
Oncology
Carcinoma
Squamous Cell
Mouth Neoplasms
medicine.symptom
Diterpenes
endocrine system
medicine.medical_specialty
9
10-Dimethyl-1
2-benzanthracene
Real-Time Polymerase Chain Reaction
stomatognathic system
Internal medicine
Proliferating Cell Nuclear Antigen
medicine
Animals
Anticarcinogenic Agents
RNA
Messenger
Cell Proliferation
Mesocricetus
7
12-Dimethylbenz[a]anthracene
Public Health
Environmental and Occupational Health
Mouth Mucosa
Neoplasms
Experimental
biology.organism_classification
Proliferating cell nuclear antigen
stomatognathic diseases
Endocrinology
chemistry
Cancer research
biology.protein
Carcinogens
Zdroj: Asian Pacific journal of cancer prevention : APJCP. 14(10)
ISSN: 2476-762X
Popis: Our aim was to explore anti-cell proliferative and anti-angiogenic potential of andrographolide by analyzing the expression pattern of cell proliferative (PCNA, Cyclin D1) and angiogenic (VEGF) markers during 7, 12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch carcinogenesis. DMBA painting three times a week for 14 weeks in the buccal pouch of golden Syrian hamsters resulted in oral tumors which were histopathologically diagnosed as well differentiated squamous cell carcinoma. Immunohistochemical (PCNA, VEGF) and RT-PCR (Cyclin D1) studies revealed over expression of PCNA, VEGF and Cyclin D1 in the buccal mucosa of hamsters treated with DMBA alone. Oral administration of andrographolide at a dose of 50 mg/kg bw to hamsters treated with DMBA not only suppressed the histological abnormalities but also down regulated the expression of PCNA, VEGF and Cyclin D1. The results of the present study suggest that andrographolide suppressed tumor formation in the buccal mucosa of hamsters treated with DMBA through its anti-cell proliferative and anti-angiogenic potential.
Databáze: OpenAIRE
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