Hepatitis C virus viremia in SCID--BNX mouse chimera
| Autor: | E. Galun, T. Burakova, M. Ketzinel, I. Lubin, E. Shezen, Y. Kahana, A. Eid, Y. Ilan, A. Rivkind, G. Pizov, D. Shouval, Y. Reisner |
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| Rok vydání: | 1995 |
| Předmět: |
Time Factors
Pan troglodytes Hepatitis C virus medicine.medical_treatment T-Lymphocytes Molecular Sequence Data Transplantation Heterologous Mice Nude Viremia Hepacivirus Mice SCID Liver transplantation Biology medicine.disease_cause Polymerase Chain Reaction Virus Lymphocyte Depletion Mice medicine Immunology and Allergy Animals Humans In Situ Hybridization Bone Marrow Transplantation DNA Primers Severe combined immunodeficiency Base Sequence Chimera Immunologic Deficiency Syndromes virus diseases Hepatitis C Total body irradiation medicine.disease Virology Liver Transplantation Rats Transplantation Disease Models Animal Infectious Diseases RNA Viral Whole-Body Irradiation |
| Zdroj: | The Journal of infectious diseases. 172(1) |
| ISSN: | 0022-1899 |
| Popis: | Chimpanzees are currently the only nonhuman animal model for reproducible propagation of hepatitis C virus (HCV). A chimeric mouse model was used for the induction of hepatitis C viremia, using BNX (beige/nude/X-linked immunodeficient) mice preconditioned by total body irradiation and reconstituted with SCID mouse bone marrow cells. HCV-infected liver fragments from patients with HCV RNA-positive sera were transplanted under the kidney capsule of the chimeric mice. HCV-specific RNA sequences were detected by reverse transcriptase nested polymerase chain reaction (RT-PCR) in serum of approximately 50% of grafted animals. In addition, normal liver specimens were incubated with HCV serum and transplanted into chimeric mice, leading to viremia in approximately 25% of animals. Sequential histologic evaluation of the liver implants, from day 2 to week 14 after transplantation, revealed loss of lobular architecture within the implants. However, viremia persisted for 10-50 days after transplantation. These results offer a new HCV model. |
| Databáze: | OpenAIRE |
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