Comparing the endothelialisation of extracellular matrix bioscaffolds with coated synthetic vascular graft materials

Autor: Pierce A. Grace, D. N. Coakley, Kathleen O'Sullivan, Eamon G. Kavanagh, Faisal M. Shaikh, Timothy M. McGloughlin, Stewart R. Walsh
Rok vydání: 2015
Předmět:
0301 basic medicine
Scaffold
medicine.medical_specialty
Swine
02 engineering and technology
Cell morphology
Extracellular matrix
03 medical and health sciences
Blood Vessel Prosthesis Implantation
Tissue engineering
Coated Materials
Biocompatible
Blood vessel prosthesis
medicine
Cell Adhesion
Human Umbilical Vein Endothelial Cells
Vascular Patency
Animals
Humans
Cell adhesion
Polytetrafluoroethylene
Cells
Cultured
Cell Proliferation
Analysis of Variance
Tissue Scaffolds
business.industry
Endothelial Cells
General Medicine
Adhesion
extracellular matrix (ECM)
021001 nanoscience & nanotechnology
Surgery
Blood Vessel Prosthesis
Extracellular Matrix
030104 developmental biology
tissue engineering
cardiovascular system
Microscopy
Electron
Scanning
endothelialisation
Endothelium
Vascular
vascular grafts
0210 nano-technology
business
biomaterials
Biomedical engineering
Zdroj: International journal of surgery (London, England). 25
ISSN: 1743-9159
Popis: Introduction Existing synthetic vascular grafts have unacceptably high failure rates when replacing below knee arteries. In vitro endothelialisation is a technique, which has been shown to enhance the patency rates of below knee vascular grafts. Synthetic materials are however poor cellular substrates and must be combined with coatings to promote cellular growth and attachment. The most common coating clinically is fibrin-coated ePTFE. The aim of our study was to compare the endothelialisation of fibrin-coated ePTFE with novel extracellular matrix (ECM) biomaterials that we hypothesise will provide a superior substrate for cell growth. Methods Human endothelial cells were cultured on ECM scaffolds and fibrin-coated ePTFE. Uncoated Dacron and ePTFE acted as controls. The cells were examined for viability, phenotype, adhesion and proliferation. Cell morphology was accessed using scanning electron microscopy. Results Cells remained viable and produced von Willebrand factor on all substrates tested. ECM scaffolds and fibrin-modified ePTFE achieved statistically higher attachment efficiency when compared to both uncoated synthetic graft materials (p ≤ 0.001). At 90 min 80 ± 3.6% of cells had attached to the ECM scaffold compared to Dacron (30 ± 4.5%, n = 3) and ePTFE (33 ± 2.5%, n = 3). There was no difference in adhesion rates between ECM scaffolds and fibrin-coated ePTFE (p = 1.00). Endothelial cells proliferated fastest on ECM scaffolds when compared to all other materials tested (p Conclusion ECM bioscaffolds offer an improved substrate for promoting rapid endothelialisation compared to fibrin-coated ePTFE by combining firm cellular anchorage and superior cell expansion.
Databáze: OpenAIRE
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