Global analysis of sRNA target genes in Mycoplasma hyopneumoniae
| Autor: | Franciele Maboni Siqueira, Tiago Ebert Fritsch, Irene Silveira Schrank |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 Swine lcsh:Biotechnology 030106 microbiology Expressão gênica Biology Genome Pequeno RNA não traduzido 03 medical and health sciences RNA interference lcsh:TP248.13-248.65 Gene expression Genetics Animals Gene Regulation of gene expression Gene Expression Profiling Mycoplasma hyopneumoniae Computational Biology Gene Expression Regulation Bacterial Pneumonia of Swine Mycoplasmal sRNA target gene Small RNA Gene expression profiling RNA Bacterial lcsh:Genetics Terminator (genetics) Heat shock Oxidative stress Reação em cadeia da polimerase em tempo real RNA Small Untranslated RNA Interference heat shock DNA microarray Transcriptome Research Article Biotechnology |
| Zdroj: | BMC Genomics, Vol 19, Iss 1, Pp 1-9 (2018) BMC Genomics Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/s12864-018-5136-5 |
| Popis: | Background Small RNAs (sRNAs) are noncoding molecules that regulate different cellular activities in several bacteria. The role of sRNAs in gene expression regulation is poorly characterized in the etiological agent of porcine enzootic pneumonia Mycoplasma hyopneumoniae. We performed a global analysis of the sRNAs, sRNA target genes and regulatory elements previously identified in their genome and analyzed the expression of some sRNAs and their target genes by quantitative RT-PCR (qPCR) in three different culture conditions. Results Seven of the 145 sRNA target genes are organized as monocistronic genes (mCs) while the other 138 sRNA target genes are organized into transcriptional units (TU). The identification of transcriptional regulatory elements (promoter motif, DNA repeat sequence or intrinsic terminator) was verified in 116 of the 145 sRNA target genes. Moreover, the 29 sRNA target genes without regulatory elements revealed the presence of at least one regulatory element in the boundaries of the TU or in other internal genes of the TU. We verified that 16 sRNAs showed differential expression, seven in heat shock condition and 14 in oxidative stress condition. Analysis of the differential expression of the sRNA target genes showed that the tested sRNAs possibly regulate gene expression. The sRNA target genes were up- or down-regulated possibly in response to sRNA only under oxidative stress condition. Moreover, the sRNA target genes are involved in diverse processes of the cell, some of which could be linked to transcription processes and cell homeostasis. Conclusion Our results indicate that bacterial sRNAs could regulate a number of targets with various outcomes, and different correlations between the levels of sRNA transcripts and their target gene mRNAs were found, which suggest that the regulation of gene expression via sRNAs may play an important role in mycoplasma. Electronic supplementary material The online version of this article (10.1186/s12864-018-5136-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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