Effect of human T lymphotropic retrovirus-I exposure on cultured human glioma cell lines
| Autor: | G. M. Lauro, Beatrice Macchi, B. Caronti, Enzo Bonmassar, Mario Pezzella |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
medicine.drug_class
viruses Immunocytochemistry Fluorescent Antibody Technique In situ hybridization Monoclonal antibody Virus Pathology and Forensic Medicine glioma HTLV-I neurotropism Cellular and Molecular Neuroscience Retrovirus Glioma Glial Fibrillary Acidic Protein Tumor Cells Cultured medicine Humans Human T-lymphotropic virus 1 biology Glial fibrillary acidic protein Settore BIO/14 Antibodies Monoclonal Nucleic Acid Hybridization biology.organism_classification medicine.disease Virology nervous system Cell culture biology.protein Neurology (clinical) |
| Popis: | Four different human tumor cell lines of glial origin have been exposed to a human T lymphotropic retrovirus (HTLV-I). All these cell lines were positive for the glial marker glial fibrillary acidic protein (GFAP). The presence of virus RNA was demonstrated by in situ hybridization using an HTLV-I, SStI-SStI viral insert as probe. Virus expression has been monitored through an indirect immunofluorescence assay using a monoclonal antibody against virus core protein p19. All the four glioma cell lines tested became positive for p19 after 2 weeks of co-cultivation and showed a clear alteration of GFAP expression. |
| Databáze: | OpenAIRE |
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