Genom‐wide analysis identifies single nucleotide polymorphism variations and altered pathways associated with poor semen quality in breeding bulls

Autor:	John Peter Ebenezer Samuel King, Arumugam Kumaresan, Thirumala Rao Talluri, Manish Kumar Sinha, Kathan Raval, Pradeep Nag, Thirumalaisamy Karuthadurai, Veeramani Aranganathan
Rok vydání:	2022
Předmět:	Adenosine Triphosphatases Male RNA Caps Hydrolases Breeding Polymorphism Single Nucleotide Spermatozoa Prolactin Semen Analysis Pyrimidines Endocrinology Purines Semen Sperm Motility Animals Cattle Animal Science and Zoology Proto-Oncogene Proteins c-akt Biomarkers Biotechnology
Zdroj:	Reproduction in Domestic Animals. 57:1143-1155
ISSN:	1439-0531 0936-6768
DOI:	10.1111/rda.14185
Popis:	The reason for poor semen quality among the breeding bulls is not well understood. In the present study, we performed high-throughput RNAseq analysis of spermatozoa to identify the SNPs present in good and poor-quality semen-producing Holstein Friesian breeding bulls. A total of 21,360 and 44,650 SNPs were identified in good and poor-quality semen with a minimum read depth of 20, among which 4780 and 8710 novel variants were observed in good and poor-quality semen, respectively. Greater SNPs and indels variations were observed in poor compared to good-quality semen. In poor-quality semen, SNP variations were observed in ZNF280B, SLC26A2, DMXL1, OR52A1, MACROD2 and REV1 genes, which are associated with regulation of spermatogenesis, post-testicular maturation, Cl
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5870c48af5222bff81dbfd603cba8ad3 https://doi.org/10.1111/rda.14185 Zobrazit plný text záznamu Plný text