Limited diagnostic possibilities for bloodstream infections with broad‐range methods: A promising PCR/electrospray ionization‐mass spectrometry platform is no longer available
| Autor: | Dragos Florea, Pavel Drevinek, Claus Moser, Jan Berousek, Jan Tkadlec, Eliska Bebrova, Tomas Vymazal, Vendula Martinkova, Jaroslava Adamkova |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Microbiological Techniques Spectrometry Mass Electrospray Ionization bloodstream infections Electrospray ionization lcsh:QR1-502 Bacteremia Mass spectrometry Diagnostic tools Polymerase Chain Reaction Microbiology lcsh:Microbiology law.invention molecular diagnostics Young Adult Broad spectrum law RNA Ribosomal 16S Sepsis medicine Humans broad‐range PCR Blood culture Time to positivity Polymerase chain reaction Aged Aged 80 and over medicine.diagnostic_test business.industry Reproducibility of Results Middle Aged Molecular diagnostics 16S PCR PCR/ESI‐MS Molecular Diagnostic Techniques Commentary Female business |
| Zdroj: | MicrobiologyOpen MicrobiologyOpen, Vol 9, Iss 5, Pp n/a-n/a (2020) |
| ISSN: | 2045-8827 |
| Popis: | Fast and accurate detection of causative agents of bloodstream infections remains a challenge of today's microbiology. We compared the performance of cutting‐edge technology based on polymerase chain reaction coupled with electrospray ionization‐mass spectrometry (PCR/ESI‐MS) with that of conventional broad‐range 16S rRNA PCR and blood culture to address the current diagnostic possibilities for bloodstream infections. Of 160 blood samples tested, PCR/ESI‐MS revealed clinically meaningful microbiological agents in 47 samples that were missed by conventional diagnostic approaches (29.4% of all analyzed samples). Notably, PCR/ESI‐MS shortened the time to positivity of the blood culture‐positive samples by an average of 34 hr. PCR/ESI‐MS technology substantially improved current diagnostic tools and represented an opportunity to make bloodstream infections diagnostics sensitive, accurate, and timely with a broad spectrum of microorganisms covered. By using the polymerase chain reaction coupled with electrospray ionization‐mass spectrometry (PCR/ESIMS) technology, we detected pathogenic agents in 29.4% (47 of 160) more samples than by using 16S rRNA PCR and/or blood culture. Thus, the PCR/ESI‐MS represented an opportunity to make the bloodstream infection diagnostics highly sensitive and accurate. |
| Databáze: | OpenAIRE |
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