Determination of Optimum Operation Parameters for Low-Intensity Pulsed Ultrasound and Low-Level Laser Based Treatment to Induce Proliferation of Osteoblast and Fibroblast Cells
| Autor: | Mehmet Emre Coskun, Kübra Açıkalın Coşkun, Yusuf Tutar |
|---|---|
| Přispěvatelé: | [Coskun, Mehmet Emre] Cumhuriyet Univ, Dept Prosthodont, Fac Dent, TR-58140 Sivas, Turkey -- [Coskun, Kubra Acikalin] Cumhuriyet Univ, Dept Biochem, Fac Pharm, Sivas, Turkey -- [Tutar, Yusuf] Univ Hlth Sci, Fac Hlth Sci, Dept Nutr & Dietet, Istanbul, Turkey |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Cell Survival Ultrasonic Therapy Biomedical Engineering Low-intensity pulsed ultrasound Radiation Dosage Sensitivity and Specificity fibroblast Application time law.invention Mice 03 medical and health sciences 0302 clinical medicine law LIPUS medicine Animals Radiology Nuclear Medicine and imaging Low-Level Light Therapy Fibroblast Cells Cultured Cell Proliferation Osteoblasts LLLT Pulse duration Osteoblast 030206 dentistry Fibroblasts Laser 030104 developmental biology medicine.anatomical_structure Ultrasonic Waves osteoblast Biomedical engineering |
| Zdroj: | Photomedicine and Laser Surgery. 36:246-252 |
| ISSN: | 1557-8550 |
| DOI: | 10.1089/pho.2017.4354 |
| Popis: | WOS: 000425757900001 PubMed ID: 29466082 Objective: The aim of this study was to determine the optimum operating parameters (pulse duration, energy levels, and application time) to promote induction of osteoblast and fibroblast cell proliferation and to maintain cell viability treated with low-intensity pulsed ultrasound (LIPUS) and low-level laser therapy (LLLT). Background data: The positive effects of LIPUS and LLLT on cellular activity have been reported in recent years. Comparisons between experimental parameters of previous studies are difficult because scientific studies reported frequencies and the duty cycles of LIPUS and wavelengths and doses of LLLT in a wide range of parameters. However, optimum amount of energy and optimum time exposure must be determined to induce bone and tissue cell proliferation for effective healing process and to avoid cell damage. Material and methods: Fibroblast and osteoblast cell cultures were irradiated with LIPUS (10-50% pulse and continuous mode at 1 and 3MHz for 1, 3, and 5min) and LLLT (4, 8, and 16J at 50, 100, 200, 300, 400, and 500mW). Cell cultures were analyzed using XTT assay. Results: For both cell types, LIPUS treatment with 10% pulse (1:9 duty cycle), 3MHz, and for 1min and LLLT treatment over 100mV for 4, 8, and 16J modalities contributed to the growth, and may help bone repair and tissue healing process optimally. Conclusions: Bio-stimulating effects of LLLT irradiation promote proliferation and maintain cell viability better than LIPUS treatment without causing thermal response for both cell types, and the therapeutic modality above 200mV has maximum effectiveness. Scientific Research Projects Unit of Cumhuriyet University, Sivas, Turkey [DIS-130] This study was funded by Scientific Research Projects Unit of Cumhuriyet University, Sivas, Turkey (Project No. DIS-130). |
| Databáze: | OpenAIRE |
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