Improved electroporation buffer enhances transient gene expression in Arachis hypogaea protoplasts
| Autor: | Ming Cheng, J. W. Demski, Robert L. Jarret, Zhijian Li |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Glycylglycine
Electroporation fungi food and beverages General Medicine biochemical phenomena metabolism and nutrition Biology Protoplast Buffer (optical fiber) Arachis hypogaea chemistry.chemical_compound chemistry Botany Glycine Gene expression Genetics medicine Biophysics Mannitol Molecular Biology Biotechnology medicine.drug |
| Zdroj: | Genome. 38:858-863 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/g95-113 |
| Popis: | An electroporation medium containing 50 mM glycine or 10 mM glycylglycine (glygly), 70 mM potassium glutamate, and 0.4 M mannitol was evaluated for its ability to improve transient β-glucuronidase (GUS) expression in immature cotyledonary protoplasts of Arachis hypogaea L. GUS activity in electroporated protoplasts was 8- to 430-fold greater than that obtained using any of other four commonly employed poration media. Analysis of viability and histochemical staining of protoplasts indicated that electroporation using the glycine- or glygly-based poration medium resulted in increased protoplast viability and GUS expression when compared with other poration media. Replacement of glygly with MES or HEPES buffers significantly reduced the level of GUS expression in electroporated protoplasts.Key words: transient expression, electroporation, Arachis, protoplasts, GUS. |
| Databáze: | OpenAIRE |
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