The Aα mating-type locus ofSchizophyflum commune: Structure and function of geneX
| Autor: | Amy L. Marion, Robert C. Ullrich, Jian Wu, Charles P. Novotny, Kirk A. Bartholomew, Huiling Yang |
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| Rok vydání: | 1996 |
| Předmět: |
Gene isoform
Mating type Genes Fungal Molecular Sequence Data Mutant Locus (genetics) Schizophyllum Biology Transformation Genetic Sequence Homology Nucleic Acid Genetics Allele Gene Alleles Base Sequence Schizophyllum commune Sequence Analysis DNA General Medicine Genes Mating Type Fungal biology.organism_classification Phenotype Blotting Southern Protein Biosynthesis Gene Targeting Mutation |
| Zdroj: | Current Genetics. 29:143-149 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/bf02221578 |
| Popis: | Gene X maps immediately right of gene Y at the A alpha mating-type locus in Schizophyllum commune. Alleles X1, X3 and X4 were isolated, subcloned, and sequenced. The structure of the alleles and their relationship to the A alpha locus is described. The deduced X isoforms possess no recognized motifs common to other polypeptides and no significant similarity to any sequences in the protein data-bases. X alleles do not activate A-regulated development when transformed into recipient strains possessing different A alpha mating types or when these transformants are mated with tester strains. An X1-disrupted construction yielded a high frequency (33%) of homologous gene replacements. X-disrupted mutants have wild-type phenotypes and mate normally. Both the functional analyses and sequence data for X1, X3, and X4 suggest that the right boundary of the A alpha mating-type locus falls between A alpha Y and X. We propose that the Z and Y genes constitute the A alpha locus in its entirety. |
| Databáze: | OpenAIRE |
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