Angiogenesis: Noninvasive Quantitative Assessment with Contrast-enhanced Functional US in Murine Model
| Autor: | Olivier Lucidarme, Robert F. Mattrey, Judith A. Varner, Sang Hee Choi, Jean Louis Golmard, Yuko Kono, Jacqueline Corbeil |
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| Přispěvatelé: | Saidi, Vanessa, Laboratoire d'Ingénierie des Protéines et Contrôle Métabolique, Centre National de la Recherche Scientifique (CNRS), Department of Radiology, University of California (UC), Department of Radiology and Center for Imaging Science, Sungkyunkwan University [Suwon] (SKKU) |
| Rok vydání: | 2006 |
| Předmět: |
Pathology
MESH: Blood Volume Time Factors Angiogenesis Basic fibroblast growth factor Contrast Media Blood volume Neovascularization MESH: Fluorocarbons Mice Random Allocation chemistry.chemical_compound MESH: Microcirculation Medicine MESH: Animals MESH: Random Alloc Skin Ultrasonography Fluorocarbons Blood Volume Microbubbles Microvascular Density Prostheses and Implants Platelet Endothelial Cell Adhesion Molecule-1 medicine.anatomical_structure MESH: Models Animal Models Animal cardiovascular system [SDV.IB]Life Sciences [q-bio]/Bioengineering Fibroblast Growth Factor 2 MESH: Image Enhancement medicine.symptom MESH: Neovascularization Physiologic Blood vessel medicine.medical_specialty MESH: Gels MESH: Prostheses and Implants Neovascularization Physiologic Microcirculation MESH: Contrast Media Animals Humans Radiology Nuclear Medicine and imaging MESH: Mice [SDV.IB] Life Sciences [q-bio]/Bioengineering MESH: Humans MESH: Fibroblast Growth Factor 2 business.industry MESH: Antigens CD31 Image Enhancement chemistry Implant business Gels MESH: Microbubbles Biomedical engineering |
| Zdroj: | Radiology Radiology, 2006, 239 (3), pp.730-9. ⟨10.1148/radiol.2392040986⟩ |
| ISSN: | 1527-1315 0033-8419 |
| DOI: | 10.1148/radiol.2392040986 |
| Popis: | PURPOSE: To evaluate quantitative functional ultrasonography (US) in a murine gel model by using microbubble destruction kinetics to determine whether parametric indices provided with US could help assess angiogenesis. MATERIALS AND METHODS: Institutional Animal Subjects Committee approved experiments and procedures. In 36 normal mice, two 0.4-mL gel implants were placed subcutaneously on either side of spine. One implant contained 0.5, 1.0, or 1.5 microg human basic fibroblast growth factor (bFGF) per milliliter of gel. Functional US quantitative analysis of angiogenesis with microbubble contrast agent was performed on days 3, 6, 9, and 12; histologic data were collected. Time-intensity curve of implant was fitted to mathematic decay model to calculate fractional blood volume and fraction of blood replaced per unit of time. Microvascular density (MVD) and percentage of microvascular area (MVA) were measured after anti-CD31 staining. Spearman rank order correlation was used in analyses. RESULTS: bFGF-containing implants induced MVD of eight, 35, 42, and 42 vessels per square millimeter on days 3, 6, 9, and 12, respectively; in controls, MVD was four vessels/mm2 (P |
| Databáze: | OpenAIRE |
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