The application of a murine bone bioreactor as a model of tumor: bone interaction
| Autor: | Lynn M. Matrisian, David Hamming, Jonathan T. Fleming, Conor C. Lynch, Herbert S. Schwartz, Ginger E. Holt, Jennifer L. Halpern, Michelle D. Martin |
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| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Bone Morphogenetic Protein 2 Bone Neoplasms Bone morphogenetic protein 2 Mice Bioreactors Transforming Growth Factor beta Osteoclast In vivo Cell Line Tumor medicine Bioreactor Animals Mammary tumor Bone Development Tissue Engineering Chemistry Osteoid technology industry and agriculture Mammary Neoplasms Experimental Osteoblast General Medicine equipment and supplies Disease Models Animal Durapatite medicine.anatomical_structure Oncology Tumor progression Bone Morphogenetic Proteins Female |
| Zdroj: | Clinical & Experimental Metastasis. 23:345-356 |
| ISSN: | 1573-7276 0262-0898 |
| Popis: | A limited number of in vivo models that rapidly assess bone development or allow for the study of tumor progression in a closed in vivo environment exist. To address this, we have used bone tissue engineering techniques to generate a murine in vivo bone bioreactor. The bioreactor was created by implanting an osteoconductive hydroxyapatite scaffold pre-loaded with saline as a control or with bone morphogenetic protein-2 (BMP-2) to the murine femoral artery. Control and BMP-2 bioreactors were harvested and histologically assessed for vascularization and bone formation at 6 and 12 weeks post implantation. BMP-2 significantly enhanced the formation of osteoid within the bioreactor in comparison to the controls. To test the in vivo bone bioreactor as a model of tumor: bone interaction, FVB mice were implanted with control or BMP-2 treated bioreactors. After 6 weeks, an osteolytic inducing mammary tumor cell line tagged with luciferase (PyMT-Luc) derived from the polyoma virus middle T (PyMT) model of mammary tumorigenesis was delivered to the bioreactor via the femoral artery. Analysis of luciferase expression over time demonstrated that the presence of osteoid in the BMP-2 treated bioreactors significantly enhanced the growth rate of the PyMT-Luc cells in comparison to the control group. These data present a unique in vivo model of ectopic bone formation that can be manipulated to address molecular questions that pertain to bone development and tumor progression in a bone environment. |
| Databáze: | OpenAIRE |
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