Controlling Nanoparticle Formulation: A Low-Budget Prototype for the Automation of a Microfluidic Platform
| Autor: | Rafał Krzysztoń, Ernst Wagner, Ulrich Lächelt, Joachim O. Rädler, Dominik M. Loy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Computer science
Microfluidics microfluidics Bioengineering 02 engineering and technology raspberry pi Fraction Collector lcsh:Chemical technology 01 natural sciences lcsh:Chemistry Hardware_INTEGRATEDCIRCUITS Chemical Engineering (miscellaneous) lcsh:TP1-1185 Macro computer.programming_language 010405 organic chemistry business.industry Process Chemistry and Technology nanoparticle Process (computing) Python (programming language) Modular design 021001 nanoscience & nanotechnology Chip Automation 0104 chemical sciences python lcsh:QD1-999 Embedded system polyplex siRNA lipoplex 0210 nano-technology business computer |
| Zdroj: | Processes, Vol 9, Iss 129, p 129 (2021) Processes Volume 9 Issue 1 |
| ISSN: | 2227-9717 |
| Popis: | Active pharmaceutical ingredients (API) with suboptimal pharmacokinetic properties may require formulation into nanoparticles. In addition to the quality of the excipients, production parameters are crucial for producing nanoparticles which reliably deliver APIs to their target. Microfluidic platforms promise increased control over the formulation process due to the decreased degrees of freedom at the micro- and nanoscale. Publications about these platforms usually provide only limited information about the soft- and hardware required to integrate the microfluidic chip seamlessly into an experimental set-up. We describe a modular, low-budget prototype for microfluidic mixing in detail. The prototype consists of four modules. The control module is a raspberry pi executing customizable python scripts to control the syringe pumps and the fraction collector. The feeding module consists of up to three commercially available, programable syringe pumps. The formulation module can be any macro- or microfluidic chip connectable to syringe pumps. The collection module is a custom-built fraction collector. We describe each feature of the working prototype and demonstrate its power with polyplexes formulated from siRNA and two different oligomers that are fed to the chip at two different stages during the assembly of the nanoparticles. |
| Databáze: | OpenAIRE |
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