Blockage of RelB expression by gene silencing enhances the radiosensitivity of androgen‑independent prostate cancer cells
| Autor: | Chun‑Hai Hu, Heng‑Cheng Zhu, Tao Qiu, Xiu‑Heng Liu, Chao Dan |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Cancer Research Apoptosis Biology Biochemistry Radiation Tolerance Prostate cancer Mice Prostate Cell Line Tumor Radiation Ionizing Genetics medicine Animals Humans Radiosensitivity RNA Small Interfering Clonogenic assay Molecular Biology Aged Oncogene RELB Transcription Factor RelB NF-kappa B Cancer Prostatic Neoplasms DNA Cell cycle Middle Aged medicine.disease Molecular biology Up-Regulation medicine.anatomical_structure Oncology Cancer research Molecular Medicine RNA Interference |
| Zdroj: | Molecular medicine reports. 11(2) |
| ISSN: | 1791-3004 |
| Popis: | Levels of the nuclear factor‑kappa B (NF‑κB) alternative pathway member RelB have been shown to correlate with the effect of radiation therapy in prostate cancer. RelB expression was evaluated by immunohistochemistry in normal prostate, benign prostate hyperplasia and prostate cancer specimens. RM‑1 cells were pretreated with RelB siRNA prior to radiation therapy, and RelB expression in cytoplasmic and nuclear extracts was detected by real‑time polymerase chain reaction and western blot analysis. The apoptotic rates of experimental RM‑1 cell groups were assessed by flow cytometry. A clonogenic growth array was used to evaluate the radiosensitivity of RM‑1 cell groups. The NF‑κB family member RelB was expressed at a high level in prostate cancer specimens. Compared with irradiated control cells, RM‑1 cells transfected with RelB siRNA and treated with radiation therapy demonstrated a significant downregulation of RelB expression in the cytoplasm and nucleus. Notably, flow cytometry revealed that pretreatment of RM‑1 cells with RelB siRNA enhanced the apoptotic rate in response to radiation therapy compared with controls. Clonogenic growth assay results revealed enhanced radiosensitivity of RelB siRNA cells at various dosage points compared with control groups. Blockage of the alternative NF‑κB pathway via RelB silencing is a promising approach to enhance the radiosensitivity of prostate cancer. |
| Databáze: | OpenAIRE |
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