Effect of bisphosphonates on the mandibular bone and gingival epithelium of rats without tooth extraction
| Autor: | Gregorio Micali, Francesco Saverio De Ponte, Giovanna Vermiglio, Giuseppina Rizzo, Giuseppina Cutroneo, Antonio Centofanti, Michele Runci, Luciano Catalfamo |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Necrosis medicine.medical_treatment Immunofluorescence mandibular bone Pathogenesis osteblasts 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) medicine Saline bisphosphonates Oosteblasts medicine.diagnostic_test business.industry Bisphosphonates Mandibular bone Gingival epithelium Oosteblasts Osteoclasts gingival epithelium 030206 dentistry General Medicine Articles Bisphosphonate medicine.disease Staining osteoclasts Apoptosis 030220 oncology & carcinogenesis medicine.symptom Osteonecrosis of the jaw business |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | Osteonecrosis of the jaw (ONJ) is an adverse effect of bisphosphonate treatment that has become the subject of increasing investigations, in particular due to its poorly understood pathogenesis. Several experimental studies on animal models have been conducted; however, the majority of these replicate human ONJ following tooth extraction, and describe alterations in the bone and gingival epithelium when necrosis is manifested. The aim of the present study was to analyze the rat mandibular bone and gingival epithelium during 45 days of zoledronate treatment (which is a bisphosphonate agent), without tooth extraction. Intraperitoneal injections of zoledronate acid (0.1 mg/kg) were performed three times a week in normal male Wistar rats (n=20), while a control group of rats (n=20) was treated with saline solution for 45 days. After 7, 15, 30 and 45 days of drug treatment, all rats were sacrificed and hematoxilin and eosin staining, immunofluorescence and scanning electron microscopy analyses were performed. The results of the analyses after 7 and 15 days of treatment were similar in the treatment and control group. After 30 and 45 days of treatment, structural alterations were observed in the bone. No structural alterations to the gingival epithelium were observed. Based on these results, it was hypothesized that low doses of zoledronate act directly on the bone tissues to induce morphological alterations from bone to necrotic tissue following surgical procedures, although no cytotoxic effects were detected in the gingival epithelium. |
| Databáze: | OpenAIRE |
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