Transformation of human cystinotic fibroblasts by SV40: characteristics of transformed cells with limited and unlimited growth potential
| Autor: | Jerry A. Schneider, Ocean L. Pellett, Robert G. Oshima, James A. Robb |
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| Rok vydání: | 1977 |
| Předmět: |
Time Factors
Physiology Clinical Biochemistry Cell Cystinosis Cystine Simian virus 40 Biology Cell Line chemistry.chemical_compound Nephropathic Cystinosis medicine Antigens Viral Cloning Cell Biology DNA Cell Transformation Viral Cell biology Clone Cells Culture Media Transformation (genetics) medicine.anatomical_structure Biochemistry chemistry Cell culture Intracellular Cell Division |
| Zdroj: | Journal of cellular physiology. 93(1) |
| ISSN: | 0021-9541 |
| Popis: | Human skin fibroblasts derived from patients with nephropathic cystinosis were transformed with SV40 virions, cloned and permitted to enter the degenerative stage of growth termed "crisis," characteristic of SV40 transformed human cells. Nephropathic cystinosis is an autosomal recessively inherited metabolic disorder resulting in the intracellular accumulation of the amino acid cystine. A transformed cystinotic cell line which was recovered from the crisis stage was indistinguishable from its transformed precrisis parental cell strain in growth rate in media containing either 1% or 10% serum, cloning efficiency on plastic, in semisolid media, or upon confluent monolayers of normal skin fibroblasts, expression of SV40 T antigen, or production of virus. However, the modal DNA content of the recovered postcrisis transformed cystinotic cell line was different from that of the cloned parental precrisis transformed cell strain, suggesting that the postcrisis line was derived from a small subpopulation of the precrisis strain. The DNA content of the established cystinotic cell line continued to be unstable during subsequent subculturing and gave rise to subclones with both more and less DNA per cell. This line now has an apparently infinite growth potential and still has the hallmark of the cystinotic parental line, the storage of abnormally large amounts of intracellular nonprotein cystine. |
| Databáze: | OpenAIRE |
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