OsVP1 activates Sdr4 expression to control rice seed dormancy via the ABA signaling pathway
| Autor: | Hu Shikai, Shaoqing Tang, Wenqiang Chen, Ju Luo, Xiangjin Wei, Gaoneng Shao, Yawen Wu, Yusong Lyu, Wei Wang, Pingliang Huang, Zhonghua Sheng, Guiai Jiao |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sdr4 Mutant Plant Science Biology 01 natural sciences lcsh:Agriculture 03 medical and health sciences lcsh:Agriculture (General) Gene Pre-harvest sprouting Oryza sativa lcsh:S Seed dormancy Wild type food and beverages Protoplast lcsh:S1-972 Cell biology 030104 developmental biology ABA Germination OsVP1 Rice Agronomy and Crop Science 010606 plant biology & botany Sprouting |
| Zdroj: | Crop Journal, Vol 9, Iss 1, Pp 68-78 (2021) |
| ISSN: | 2214-5141 |
| DOI: | 10.1016/j.cj.2020.06.005 |
| Popis: | Pre-harvest sprouting (PHS) is a disadvantageous trait in cereal production worldwide, causing large economic losses each year. Its regulation mechanism is still unclear. We generated the Oryza sativa Viviparous1 (OsVP1) mutant using gene editing technique, which shows increased PHS compared with that of the wild type Nipponbare. OsVP1 is localized mainly in the nucleus and expressed in various tissues and organs. Expression of Seed dormancy 4 (Sdr4), a key gene controlling PHS, was sharply reduced in OsVP1 mutants. OsVP1 bound to the specific motif CACCTG in the promoter of Sdr4 and activated its expression in rice protoplasts. Overexpression of Sdr4 reduced the high seed germination rate of OsVP1 mutant cr-osvp1-1, showing that Sdr4 acts as a downstream target of OsVP1. Both OsVP1 and Sdr4 loss-of-function mutants were insensitive to exogenous ABA and employed the ABA signaling pathway in regulating seed dormancy. These findings shed light on the control of seed dormancy aimed at preventing PHS in rice. |
| Databáze: | OpenAIRE |
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