Regulation of the Bacillus subtilis acetate kinase gene by CcpA
| Autor: | D. A. Waters, Tina M. Henkin, Frank J. Grundy, S. H. G. Allen |
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| Rok vydání: | 1993 |
| Předmět: |
Mutant
Molecular Sequence Data Restriction Mapping Repressor Bacillus subtilis Biology medicine.disease_cause Microbiology Gene Expression Regulation Enzymologic chemistry.chemical_compound Open Reading Frames Bacterial Proteins Gene expression medicine Escherichia coli Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Molecular Biology Acetate kinase Base Sequence Sequence Homology Amino Acid Acetate Kinase Gene Expression Regulation Bacterial biology.organism_classification Molecular biology DNA-Binding Proteins Repressor Proteins Kinetics Glucose Biochemistry chemistry Regulatory sequence Genes Bacterial CCPA Research Article |
| Zdroj: | Journal of bacteriology. 175(22) |
| ISSN: | 0021-9193 |
| Popis: | The Bacillus subtilis gene encoding acetate kinase was identified on the basis of sequence similarity to the Escherichia coli ackA gene and to a second E. coli gene closely related to ackA. Insertional inactivation of this region of the B. subtilis chromosome resulted in the disappearance of acetate kinase enzyme activity in cell extracts. The ackA gene was mapped to a site close to the ccpA gene, at 263 degrees. The transcriptional start site for B. subtilis ackA was located 90 bp upstream from the start of the coding region, and expression was increased by growth in the presence of excess glucose. Growth of the AckA- mutant was inhibited by glucose, suggesting that acetate kinase is important for excretion of excess carbohydrate. The stimulation of ackA expression by glucose was blocked in a CcpA- mutant, indicating that CcpA, which is required for glucose repression of certain carbon source utilization genes, including amyE, may also be involved in activation of carbon excretion pathways. Two sequences resembling the amyO operator site were identified upstream of the ackA promoter; removal of this region resulted in loss of glucose activation of ackA expression. |
| Databáze: | OpenAIRE |
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