The influence of five metallic nanoparticles on the expression of major drug-metabolizing enzyme genes with correlation of inflammation in mouse livers
Autor: | Ali A. Shati, Amin A Al-Doaiss, Bashir M. Jarrar, Yazun Jarrar, Mohammad Y. Alfaifi, Mohammed A. Al-Kahtani |
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Rok vydání: | 2020 |
Předmět: |
inorganic chemicals
Male Silicon dioxide Metabolic Clearance Rate Health Toxicology and Mutagenesis chemistry.chemical_element Down-Regulation Gene Expression Metal Nanoparticles Inflammation Zinc 010501 environmental sciences Toxicology 01 natural sciences law.invention 03 medical and health sciences chemistry.chemical_compound law mental disorders Gene expression medicine Animals Cytochrome P-450 CYP3A Interleukin 6 Gene health care economics and organizations Polymerase chain reaction 030304 developmental biology 0105 earth and related environmental sciences Cytochrome P-450 CYP2C9 Pharmacology chemistry.chemical_classification 0303 health sciences Mice Inbred BALB C biology Interleukin-6 technology industry and agriculture General Medicine respiratory system Molecular biology Enzyme chemistry Liver biology.protein Environmental Pollutants medicine.symptom |
Zdroj: | Environmental toxicology and pharmacology. 80 |
ISSN: | 1872-7077 |
Popis: | Metallic nanoparticles (NPs) are widely used in medical preparations. The present study aims to find out the influence of widely used five metallic NPs on the expression of major hepatic drug-metabolizing enzyme (DME) genes. Six groups of BALB/C mice, 7 mice each, were exposed to: Gold NPs, silver NPs, copper oxide NPs, silicon dioxide NPs and zinc oxide NPs, for 21 days. Liver biopsies from all mice were subjected to mouse cyp3a11, cyp2c29, ugt2b1 and interleukin-6 (il6) gene expression quantification using real-time polymerase chain reaction, in addition to inflammatory cell infiltration examination. All tested NPs caused a sharp and significant (ANOVA, p value0.05) downregulation in the expression of DME genes, with the highest influence was observed in mice exposed to copper oxide NPs. Additionally, all NPs induced hepatic inflammation and upregulated the expression of il6 gene, which were inversely correlated with the expression of DMEs. It is concluded that all tested NPs downregulated the expression of DME genes, with the highest influence exhibited by copper oxide NPs, in correlation with inflammation and il6 gene induction in the liver. Further studies are needed to find out the effect of anti-inflammatory compounds against the alterations induced by metallic NPs exposure on hepatic DMEs. |
Databáze: | OpenAIRE |
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