The influence of five metallic nanoparticles on the expression of major drug-metabolizing enzyme genes with correlation of inflammation in mouse livers

Autor: Ali A. Shati, Amin A Al-Doaiss, Bashir M. Jarrar, Yazun Jarrar, Mohammad Y. Alfaifi, Mohammed A. Al-Kahtani
Rok vydání: 2020
Předmět:
inorganic chemicals
Male
Silicon dioxide
Metabolic Clearance Rate
Health
Toxicology and Mutagenesis
chemistry.chemical_element
Down-Regulation
Gene Expression
Metal Nanoparticles
Inflammation
Zinc
010501 environmental sciences
Toxicology
01 natural sciences
law.invention
03 medical and health sciences
chemistry.chemical_compound
law
mental disorders
Gene expression
medicine
Animals
Cytochrome P-450 CYP3A
Interleukin 6
Gene
health care economics and organizations
Polymerase chain reaction
030304 developmental biology
0105 earth and related environmental sciences
Cytochrome P-450 CYP2C9
Pharmacology
chemistry.chemical_classification
0303 health sciences
Mice
Inbred BALB C
biology
Interleukin-6
technology
industry
and agriculture
General Medicine
respiratory system
Molecular biology
Enzyme
chemistry
Liver
biology.protein
Environmental Pollutants
medicine.symptom
Zdroj: Environmental toxicology and pharmacology. 80
ISSN: 1872-7077
Popis: Metallic nanoparticles (NPs) are widely used in medical preparations. The present study aims to find out the influence of widely used five metallic NPs on the expression of major hepatic drug-metabolizing enzyme (DME) genes. Six groups of BALB/C mice, 7 mice each, were exposed to: Gold NPs, silver NPs, copper oxide NPs, silicon dioxide NPs and zinc oxide NPs, for 21 days. Liver biopsies from all mice were subjected to mouse cyp3a11, cyp2c29, ugt2b1 and interleukin-6 (il6) gene expression quantification using real-time polymerase chain reaction, in addition to inflammatory cell infiltration examination. All tested NPs caused a sharp and significant (ANOVA, p value0.05) downregulation in the expression of DME genes, with the highest influence was observed in mice exposed to copper oxide NPs. Additionally, all NPs induced hepatic inflammation and upregulated the expression of il6 gene, which were inversely correlated with the expression of DMEs. It is concluded that all tested NPs downregulated the expression of DME genes, with the highest influence exhibited by copper oxide NPs, in correlation with inflammation and il6 gene induction in the liver. Further studies are needed to find out the effect of anti-inflammatory compounds against the alterations induced by metallic NPs exposure on hepatic DMEs.
Databáze: OpenAIRE
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