Molecular detection of Mycobacterium tuberculosis sensitivity to rifampicin and isoniazid in South Gondar Zone, northwest Ethiopia
Autor: | Gobena Ameni, Temesgen Mohammed, Samuel Tolosa, Amir Alelign, Aboma Zewude, Beyene Petros |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Tuberculosis 030106 microbiology Antitubercular Agents Northwest Ethiopia Microbial Sensitivity Tests Drug resistance lcsh:Infectious and parasitic diseases Mycobacterium tuberculosis 03 medical and health sciences 0302 clinical medicine Medical microbiology Tuberculosis Multidrug-Resistant Genotype M. tuberculosis medicine Humans lcsh:RC109-216 030212 general & internal medicine biology business.industry Isoniazid South Gondar biology.organism_classification medicine.disease Virology Molecular Typing Infectious Diseases Parasitology business Rifampicin Drug sensitivity medicine.drug |
Zdroj: | BMC Infectious Diseases, Vol 19, Iss 1, Pp 1-8 (2019) |
ISSN: | 1471-2334 |
DOI: | 10.1186/s12879-019-3978-3 |
Popis: | Background Drug resistant tuberculosis (TB) has become a persistent health threat in Ethiopia. In this respect, baseline data are scarce in many parts of high TB burden regions including the different zones of Ethiopia. Methods A total of 111 culture positive M. tuberculosis isolates were recovered from TB patients and identified using region of difference (RD) 9 based polymerase chain reaction (PCR) and spoligotyping. Thereafter, their drug sensitivities to Rifampicin (RIF) and Isoniazid (INH) were evaluated using GenoType MTBDRplus assay. Results The result showed that 18.0% (20/111) of the isolates were resistant to either RIF or INH. Furthermore, 16.7 and 23.8% of the isolates from new and retreatment cases were resistant to any of the two anti-TB drugs, respectively. Multi-drug resistant (MDR) TB was detected on 1.8% (2/111) of all cases. Significantly higher frequencies of any drug resistance were observed among Euro-American (EA) major lineage (χ2: 9.67; p = 0.046). Conclusion Considerably high proportion of drug resistant M. tuberculosis strains was detected which could suggest a need for an increased effort to strengthen TB control program in the study area. |
Databáze: | OpenAIRE |
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