A tropomyosin-like Meretrix meretrix Linnaeus polypeptide inhibits the proliferation and metastasis of glioma cells via microtubule polymerization and FAK/Akt/MMPs signaling
| Autor: | Changhui Wang, Ning Wu, Quanbin Zhang, Zhongjun Fan, Xiukun Lin, Qi Xu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cell cycle checkpoint
Mice Nude Antineoplastic Agents Apoptosis 02 engineering and technology Tropomyosin Matrix metalloproteinase Biochemistry Microtubules Microtubule polymerization Polymerization 03 medical and health sciences Mice Structural Biology In vivo Cell Movement Cell Line Tumor Animals Humans Molecular Biology Protein kinase B 030304 developmental biology Cell Proliferation 0303 health sciences Chemistry General Medicine Cell Cycle Checkpoints Glioma 021001 nanoscience & nanotechnology HCT116 Cells Xenograft Model Antitumor Assays In vitro Matrix Metalloproteinases nervous system diseases Cancer research MCF-7 Cells NIH 3T3 Cells 0210 nano-technology Glioblastoma Peptides Proto-Oncogene Proteins c-akt HeLa Cells Signal Transduction |
| Zdroj: | International journal of biological macromolecules. 145 |
| ISSN: | 1879-0003 |
| Popis: | Glioblastoma (GBM) represents the most common, aggressive and deadliest primary tumors with poor prognosis as available therapeutic approaches fail to control its aberrant proliferation and high invasiveness. Thus, the therapeutic agents targeting these two characteristics will be more effective. In present study, a novel polypeptide (MM15), which was originally purified from Meretrix meretrix Linnaeus and has been proven to possess potent antitumor activity by our laboratory, was recombinant expressed and identified as a tropomyosin homologous protein. The recombinant polypeptide (re-MM15) could induce the U87 cell cycle arrest in G2/M phase and cell apoptosis by inducing tubulin polymerization. Additionally, re-MM15 displayed the significant inhibition to the migration and invasion of U87 cells through downregulating FAK/Akt/MMPs signaling. Furthermore, the in vivo analysis suggested that re-MM15 significantly blocked tumor growth in U87 xenograft model. Collectively, our results indicated that re-MM15, with anti-GBM properties in vitro and in vivo, has promising potential as a new anticancer candidate for GBM. |
| Databáze: | OpenAIRE |
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