Antibodies Against Hepatitis C Virus–Like Particles and Viral Clearance in Acute and Chronic Hepatitis C
| Autor: | Hubert E. Blum, Johnson Y.N. Lau, Shigeaki Aono, T. Jake Liang, David Herion, Gerd R. Pape, J. Tilman Gerlach, Sabine Wellnitz, Jay H. Hoofnagle, Thomas F. Baumert, Jujin Satoi |
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| Rok vydání: | 2000 |
| Předmět: |
Hepatitis C virus
Alpha interferon Enzyme-Linked Immunosorbent Assay Hepacivirus medicine.disease_cause Antiviral Agents Virus-like particle Interferon medicine Humans Interferon alfa Hepatology biology Virion Interferon-alpha virus diseases Hepatitis C Hepatitis C Antibodies Hepatitis C Chronic medicine.disease Virology digestive system diseases Acute Disease Immunology Humoral immunity biology.protein Antibody medicine.drug |
| Zdroj: | Hepatology. 32:610-617 |
| ISSN: | 1527-3350 0270-9139 |
| Popis: | We recently described the efficient assembly of hepatitis C virus (HCV) structural proteins into HCV-like particles (HCV-LPs) in insect cells. These noninfectious HCV-LPs have similar morphologic and biophysical properties as putative virions isolated from HCV-infected humans and can induce a broadly directed immune response in animal models. The HCV envelope proteins of HCV-LPs are presumably presented in a native, virion-like conformation and may therefore interact with antienvelope antibodies directed against conformational epitopes. In this study, HCV-LPs were used as capture antigens in an enzyme-linked immunosorbent assay (ELISA) to detect and quantify antibodies against HCV structural proteins in patients with acute and chronic hepatitis C. High titers of anti–HCV-LP antibodies were detected in patients chronically infected with HCV genotypes 1 to 6. In contrast to individuals with chronic hepatitis C, patients with acute self-limited hepatitis C displayed only a transient and weak seroreactivity against HCV-LPs. Patients with chronic HCV infection successfully treated with interferon demonstrated a gradual decline of anti–HCV-LP titers during or subsequent to viral clearance. Sustained interferon responders were characterized by significantly higher pretreatment levels of anti–HCV-LP antibodies as compared with nonresponders (P = .0001). In conclusion, HCV infection is associated with limited humoral immunity against the envelope proteins present on the HCV-LPs. An HCV-LP–based ELISA may be a useful diagnostic tool to distinguish acute hepatitis C from chronic HCV infection with exacerbation, and to predict viral clearance in response to interferon. (Hepatology 2000;32:610-617.) |
| Databáze: | OpenAIRE |
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