Correlation between temperature-stable E-rosette formation and lymphocyte commitment of activation
| Autor: | Ellen R. Richie, Myra Patchen |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Rosette Formation
biology DNA synthesis Lymphoblast Lymphocyte Immunology Temperature Lectin Stimulation DNA Lymphocyte Activation Methylmannosides Molecular biology Wheat germ agglutinin Pathology and Forensic Medicine Transformation (genetics) medicine.anatomical_structure Biochemistry Concanavalin A biology.protein medicine Humans Immunology and Allergy Phytohemagglutinins |
| Zdroj: | Clinical Immunology and Immunopathology. 11:88-97 |
| ISSN: | 0090-1229 |
| DOI: | 10.1016/0090-1229(78)90206-4 |
| Popis: | Human peripheral blood T lymphocytes acquire the capacity to form temperature-stable sheep erythrocyte (E) rosettes within 1 hr following phytohemagglutinin (PHA) stimulation. Whereas lymphocyte interaction with PHA or concanavalin A (Con A) induces early membrane-associated events allowing E-rosette formation at 37 as well as 4°C, wheat germ agglutinin, a nonmitogenic, erythroagglutinating lectin, is unable to effect a similar membrane change. Only mitogenic concentrations of PHA induce temperature-stable rosette-forming cells although this phenomenon occurs prior to lymphoblast transformation and is independent of DNA synthesis per se. The capacity to bind E at 37°C parallels cellular commitment to activation. Interference with Con A-mediated lymphocyte activation by addition of α-methyl- d -mannoside within several hours after culture initiation inhibits both rosette formation at 37°C and DNA synthesis to a comparable degree. |
| Databáze: | OpenAIRE |
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