Effects of tooth storage media on periodontal ligament preservation
| Autor: | Nobuko Obara, Riasat Hasan, Takashi Saito, Kazuharu Irie, Nazmus Shalehin, Hiroaki Takebe |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival Periodontal Ligament Organ Preservation Solutions Dentistry Balanced salt solution Immunoenzyme Techniques Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine stomatognathic system Egg White Ankylosis medicine Periodontal fiber Animals Dental alveolus Chemistry business.industry 030206 dentistry medicine.disease Tooth Avulsion Rats Transplantation stomatognathic diseases 030104 developmental biology Milk Rat molar Oral Surgery Isotonic Solutions business Egg white |
| Zdroj: | Dental traumatology : official publication of International Association for Dental Traumatology. 33(5) |
| ISSN: | 1600-9657 |
| Popis: | Background / Aims An easily available tooth storage medium is required to preserve a tooth after avulsion. Milk and Hank's balanced salt solution (HBSS) are recommended as tooth storage media, and egg white is also reported to be comparable with milk. The aim of the present study was to histologically and immunohistochemically evaluate the effect of different tooth storage media on the periodontal ligament (PDL) of extracted teeth. Material and Methods This experiment used HBSS, milk and egg white as tooth storage media. A total of ninety-six 6-week-old male Sprague-Dawley rats were used in these experiments. In each experiment, six rats were used for each medium and for the control group. Extracted rat molar teeth were immersed in these three different storage media for one-hour. In each medium, six samples (n=18) were fixed immediately, and the remaining samples (n= 54) were subcutaneously transplanted. In the control group (n=24), the extracted teeth were fixed or transplanted immediately after extraction. At day four, one week and two weeks after transplantation, the teeth were examined by radiographic, histological and immunohistochemical methods. The number of PDL cells in the storage media was also counted. Results Teeth immersed for one-hour in milk showed the thinnest PDL. Immunohistochemistry of periostin and CD68 labeling suggested degradation of the extracellular matrix in the PDL. In the media used for immersion, more PDL cells were observed in milk than in the other solutions. After transplantation, the HBSS and egg white groups maintained adequate thickness of PDLbut in the milk group, thinner PDL and ankylosis were observed. Conclusion Adequate thickness of PDL was maintained in the egg white group. Whereas, the milk group showed disturbance in the PDL, which may lead to ankylosis. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text