Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents
| Autor: | Claudio Rivera, Ekaterina Mugantseva, Heikki Rauvala, Ilya Kirilkin, Rashid Giniatullin, Dmitry Molotkov, Dmytro Toptunov, Leonard Khiroug, Julia Kolikova, Stanislav Khirug, Vootele Voikar, Mikhail Kislin, Mikhail Yuryev, Evgeny Pryazhnikov, Natalia Kulesskaya |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
General anesthetics Computer science dendrites General Chemical Engineering Neuroimaging Virtual reality General Biochemistry Genetics and Molecular Biology blood vessels 03 medical and health sciences intrinsic optical imaging Mice 0302 clinical medicine Ca2+ imaging Human–computer interaction Animals Empty Value Habituation Novel object recognition 030304 developmental biology Issue 88 awake 0303 health sciences Microscopy in vivo two-photon microscopy General Immunology and Microbiology Behavior Animal General Neuroscience Head fixation dendritic spines patch-clamp Electrophysiology Microscopic imaging Female Single-Cell Analysis Neuroscience 030217 neurology & neurosurgery Craniotomy |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| Popis: | It is widely acknowledged that the use of general anesthetics can undermine the relevance of electrophysiological or microscopical data obtained from a living animal’s brain. Moreover, the lengthy recovery from anesthesia limits the frequency of repeated recording/imaging episodes in longitudinal studies. Hence, new methods that would allow stable recordings from non-anesthetized behaving mice are expected to advance the fields of cellular and cognitive neurosciences. Existing solutions range from mere physical restraint to more sophisticated approaches, such as linear and spherical treadmills used in combination with computer-generated virtual reality. Here, a novel method is described where a head-fixed mouse can move around an air-lifted mobile homecage and explore its environment under stress-free conditions. This method allows researchers to perform behavioral tests (e.g., learning, habituation or novel object recognition) simultaneously with two-photon microscopic imaging and/or patch-clamp recordings, all combined in a single experiment. This video-article describes the use of the awake animal head fixation device (mobile homecage), demonstrates the procedures of animal habituation, and exemplifies a number of possible applications of the method. |
| Databáze: | OpenAIRE |
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