Pretreatment with the probiotic VSL#3 delays transition from inflammation to dysplasia in a rat model of colitis-associated cancer
| Autor: | Angel A. Isidro, Caroline B. Appleyard, Janelle C. Arthur, Claudio De Simone, Myrella L Cruz, Christian Jobin |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Colon Physiology Colorectal cancer Inflammation Gastroenterology Calcitriol receptor Rats Sprague-Dawley Physiology (medical) Internal medicine medicine Carcinoma Animals Colitis Angiostatins Cancer Biology Hepatology business.industry Probiotics Streptococcus Cancer Alkaline Phosphatase medicine.disease Endostatins Rats Disease Models Animal Lactobacillus Dysplasia Chronic Disease Immunology Metagenome Receptors Calcitriol Alkaline phosphatase Bifidobacterium medicine.symptom Colorectal Neoplasms business Precancerous Conditions |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 301:G1004-G1013 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.00167.2011 |
| Popis: | Evidence supports involvement of microflora in the transition of chronic inflammation to neoplasia. We investigated the protective efficacy of the probiotic VSL#3 in a model of colitis-associated colorectal cancer. Chronic colitis was induced in Sprague-Dawley rats by administration of trinitrobenzene sulfonic acid (TNBS), followed 6 wk later by systemic reactivation. To induce colitis-associated dysplasia and cancer, the animals received TNBS (intravenously) twice a week for 10 wk. One group received VSL#3 in drinking water from 1 wk before colitis induction until death. The colons were examined for damage and presence of dysplasia or cancer. Samples were analyzed for cell proliferation and apoptosis, vitamin D receptor (VDR) expression, angiogenic factors, and presence of alkaline sphingomyelinase or phosphatase. Microbial community composition was evaluated by terminal restriction fragment-length polymorphism analysis of the bacterial 16S rRNA gene. None of the probiotic-treated animals developed carcinoma, and no high-grade dysplasia was found in either the proximal or mid colon. In contrast, 29% of the animals in the control group developed carcinoma in one or more regions of the colon. VSL#3-treated animals had significantly less damage than the vehicle treated-controls in all areas of the colon, and this correlated with decreased richness and diversity of the mucosally adherent microbiota. Treatment with the probiotic increased the antiangiogenic factor angiostatin, VDR expression, and alkaline sphingomyelinase. We concluded that pretreatment with the probiotic VSL#3 can attenuate various inflammatory-associated parameters, delaying transition to dysplasia and cancer, thus offering its potential therapeutic use in patients with long-standing colitis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|