Infection-Derived Enterococcus faecalis Strains Are Enriched in esp , a Gene Encoding a Novel Surface Protein
| Autor: | Mark M. Huycke, Gunnar Lindahl, Arto S. Baghdayan, Michael S. Gilmore, Viswanathan Shankar |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Adolescent Molecular Sequence Data Immunology Biology Microbiology Enterococcus faecalis Conserved sequence fluids and secretions Bacterial Proteins Humans Amino Acid Sequence Child Gene Peptide sequence Conserved Sequence Gram-Positive Bacterial Infections Regulation of gene expression Antigens Bacterial Structural gene Protein primary structure Genetic Variation Membrane Proteins Bacterial Infections Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification Clone Cells Infectious Diseases Membrane protein Genes Bacterial Tandem Repeat Sequences Child Preschool Antigens Surface Parasitology |
| Zdroj: | Infection and Immunity. 67:193-200 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | We report the identification of a new cell wall-associated protein of Enterococcus faecalis . Studies on the distribution of the gene encoding this novel surface protein, Esp, reveal a significant ( P < 0.001) enrichment in infection-derived E. faecalis isolates. Interestingly, the esp gene was not identified in any of 34 clinical E. faecium isolates or in 4 other less pathogenic enterococcal species tested. Analysis of the structural gene among various E. faecalis isolates reveals the existence of alternate forms of expression of the Esp protein. The deduced primary structure of the Esp protein from strain MMH594, inferred to be 1,873 amino acids (aa) with a predicted mass of ∼202 kDa, reveals a core region consisting of repeat units that make up 50% of the protein. Esp bears global organizational similarity to the Rib and C alpha proteins of group B streptococci. Identity among Esp, Rib, and C alpha proteins is strikingly localized to a stretch of 13 aa within repeats of similar length. The high degree of conservation of this 13-residue sequence suggests that it plays an important role in the natural selection for this trait among infection-derived E. faecalis and group B streptococcal isolates. |
| Databáze: | OpenAIRE |
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