Comparison of the Structural Characteristics of Native Collagen Fibrils Derived from Bovine Tendons Using Two Different Methods: Modified Acid-Solubilized and Pepsin-Aided Extraction
| Autor: | Gang Zhang, Xiu-Ying Liu, Yongsheng Yang, Dezheng Liu, Hai-Yan Ju |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Circular dichroism
Morphology (linguistics) Sodium chemistry.chemical_element 02 engineering and technology 010402 general chemistry Fibril 01 natural sciences lcsh:Technology Article Crystallinity pepsin-aided extraction morphology General Materials Science type i collagen fibrils lcsh:Microscopy lcsh:QC120-168.85 Gel electrophoresis Chromatography lcsh:QH201-278.5 Chemistry aggregation structure lcsh:T Extraction (chemistry) 021001 nanoscience & nanotechnology acid-solubilized method 0104 chemical sciences lcsh:TA1-2040 lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering 0210 nano-technology lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 Type I collagen |
| Zdroj: | Materials, Vol 13, Iss 2, p 358 (2020) Materials Volume 13 Issue 2 |
| ISSN: | 1996-1944 |
| Popis: | Native collagen fibrils (CF) were successfully extracted from bovine tendons using two different methods: modified acid-solubilized extraction for A-CF and pepsin-aided method for P-CF. The yields of A-CF and P-CF were up to 64.91% (± 1.07% SD) and 56.78% (± 1.22% SD) (dry weight basis), respectively. The analyses of both amino acid composition and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that A-CF and P-CF were type I collagen fibrils. Both A-CF and P-CF retained the intact crystallinity and integrity of type I collagen&rsquo s natural structure by FTIR spectra, circular dichroism spectroscopy (CD) and X-ray diffraction detection. The aggregation structures of A-CF and P-CF were displayed by UV&ndash Vis. However, A-CF showed more intact aggregation structure than P-CF. Microstructure and D-periodicities of A-CF and P-CF were observed (SEM and TEM). The diameters of A-CF and P-CF are about 386 and 282 nm, respectively. Although both A-CF and P-CF were theoretically concordant with the Schmitt hypothesis, A-CF was of evener thickness and higher integrity in terms of aggregation structure than P-CF. Modified acid-solubilized method provides a potential non-enzyme alternative to extract native collagen fibrils with uniform thickness and integral aggregation structure. |
| Databáze: | OpenAIRE |
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