Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development
| Autor: | Jean-Denis Benazet, Andrew P. McMahon, Elisabeth A. Rutledge |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
In silico Organogenesis Population RNA-Seq Biology Kidney 03 medical and health sciences Mice 0302 clinical medicine Techniques and Resources Gene expression Animals Humans Stem Cell Niche education Molecular Biology Gene In Situ Hybridization Progenitor Genetics education.field_of_study Sequence Analysis RNA Gene Expression Regulation Developmental Cell biology 030104 developmental biology Organ Specificity Ureteric bud Ureter 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Development (Cambridge, England). 144(17) |
| ISSN: | 1477-9129 |
| Popis: | Branching morphogenesis creates arborized epithelial networks. In the mammalian kidney, an epithelial progenitor pool at ureteric branch tips (UBT) creates the urine-transporting collecting system. Using region-specific mouse reporter strains, we performed an RNA-seq screen, identifying tip and stalk enriched gene sets in the developing collecting duct system. Detailed in situ hybridization studies of tip-enriched predictions identified UBT-enriched gene sets conserved between the mouse and human kidney. Comparative spatial analysis of their UBT niche expression highlighted distinct patterns of gene expression revealing novel molecular heterogeneity within the UBT progenitor population. To identify kidney-specific and shared programs of branching morphogenesis, comparative expression studies on the developing mouse lung were combined with in silico analysis of the developing mouse salivary gland. These studies highlight a shared gene set with multi-organ tip enrichment and a gene set specific to UBTs. This comprehensive analysis extends our current understanding of the ureteric branch tip niche. |
| Databáze: | OpenAIRE |
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