Direct Sensitivity Test of the MB/BacT System
| Autor: | Paulo Cesar de Souza Caldas, Reginalda Ferreira de Melo Medeiros, Joselba Borges Melo Araujo, Angela Maria Werneck Barreto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Antitubercular Agents lcsh:QR1-502 Microbial Sensitivity Tests Sensitivity and Specificity lcsh:Microbiology Microbiology chemistry.chemical_compound Trisodium phosphate laboratory diagnostics Confidence Intervals medicine Humans Ethambutol Chromatography drug resistance business.industry Isoniazid Becton dickinson Mycobacterium tuberculosis Pyrazinamide bacterial infections and mycoses chemistry tuberculosis Streptomycin Sputum Ethionamide Reagent Kits Diagnostic medicine.symptom business medicine.drug |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 97, Iss 2, Pp 263-264 (2002) Memórias do Instituto Oswaldo Cruz, Volume: 97, Issue: 2, Pages: 263-264, Published: MAR 2002 |
| ISSN: | 1678-8060 0074-0276 |
| Popis: | In order to evaluate the direct-method test of sensitivity to drugs used in the principal tuberculosis treatment regimes, in the Organon Teknika MB/BacT system, we tested 50 sputum samples positive to microscopy taken from patients with pulmonary tuberculosis and with clinical indications for an antibiogram, admitted sequentially for examination during the routine of the reference laboratory. The material was treated v/v with 23% trisodium phosphate solution, incubated for 24 h at 35 degrees C, and neutralized v/v with 20% monosodium phosphate solution. The material was then centrifuged and the sediment inoculated into flasks containing Rifampin - 2 micro g/ml, Isoniazid - 0.2 micro g/ml, Pyrazinamide - 100 micro g/ml, Ethambutol - 2.5 micro g/ml, Ethionamide - 1.25 micro g/ml, and Streptomycin - 2 micro g/ml. The tests were evaluated using the indirect method in the BACTEC 460 TB (Becton Dickinson) system as the gold standard. The results showed that the Rifampin test performed best, i.e., 100% sensitivity at 95% Confidence Interval (82.2-100) and 100% specificity at 95% Confidence Interval (84.5-100), followed by Isoniazid and Pyrazinamide. In this experiment, 92% of the materials showed a final reading in 30 days; this period represents the time for primary isolation as well as the results of the sensitivity profile, and is within Centers for Disease Control and Prevention recommendations regarding time for performance of the antibiogram. The inoculated flasks showed no contamination during the experiment. The MB/BacT is shown to be a reliable, rapid, fully automated nonradiometric system for the tuberculosis antibiogram. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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