Dcp1-bodies in mouse oocytes
| Autor: | Adam Swetloff, Jean-Dominique Vassalli, Jean-Luc Pitetti, Serge Nef, Béatrice Conne, Joachim Huarte |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Endoribonucleases/genetics/ metabolism
RNA-binding protein Cytoplasmic Granules/ metabolism Recombinant Fusion Proteins/genetics/metabolism Ribonucleoproteins/genetics/metabolism DEAD-box RNA Helicases Mice Gene expression Protein biosynthesis Cycloheximide RNA Small Interfering ddc:616 Protein Synthesis Inhibitors RNA-Binding Proteins Articles RNA Helicase A Cell biology Meiosis medicine.anatomical_structure Protein Synthesis Inhibitors/pharmacology Ribonucleoproteins Female Puromycin Germ cell Meiosis/physiology Protein Biosynthesis/drug effects DEAD-box RNA Helicases/genetics/metabolism Recombinant Fusion Proteins RNA Small Interfering/genetics/metabolism Mice Transgenic Biology Cytoplasmic Granules Cell Line Oocytes/cytology/drug effects/ physiology Proto-Oncogene Proteins Endoribonucleases medicine Gene silencing Animals Humans RNA-Binding Proteins/genetics/metabolism Molecular Biology Messenger RNA RNA Proto-Oncogene Proteins/genetics/metabolism Cell Biology Trans-Activators/genetics/ metabolism Molecular biology Mice Inbred C57BL Puromycin/pharmacology Protein Biosynthesis Oocytes Trans-Activators Cycloheximide/pharmacology |
| Zdroj: | Molecular Biology of the Cell, Vol. 20, No 23 (2009) pp. 4951-4961 |
| ISSN: | 1939-4586 1059-1524 |
| Popis: | Processing bodies (P-bodies) are cytoplasmic granules involved in the storage and degradation of mRNAs. In somatic cells, their formation involves miRNA-mediated mRNA silencing. Many P-body protein components are also found in germ cell granules, such as in mammalian spermatocytes. In fully grown mammalian oocytes, where changes in gene expression depend entirely on translational control, RNA granules have not as yet been characterized. Here we show the presence of P-body-like foci in mouse oocytes, as revealed by the presence of Dcp1a and the colocalization of RNA-associated protein 55 (RAP55) and the DEAD box RNA helicase Rck/p54, two proteins associated with P-bodies and translational control. These P-body-like structures have been called Dcp1-bodies and in meiotically arrested primary oocytes, two types can be distinguished based on their size. They also have different protein partners and sensitivities to the depletion of endogenous siRNA/miRNA and translational inhibitors. However, both type progressively disappear during in vitro meiotic maturation and are virtually absent in metaphase II–arrested secondary oocytes. Moreover, this disassembly of hDcp1a-bodies is concomitant with the posttranslational modification of EGFP-hDcp1a. |
| Databáze: | OpenAIRE |
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