Role of macrophage secretions on rat polycystic ovary: Its effect on apoptosis

Autor: Myriam Forneris, Liliana Oliveros, Mariano Acosta, Florencia Figueroa, Alicia Motta, Fabian Mohamed
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Embryology
medicine.medical_treatment
Nitric Oxide Synthase Type II
Apoptosis
Immunoenzyme Techniques
purl.org/becyt/ford/1 [https]
Endocrinology
Prostaglandin E2
MACROPHAGES
Cells
Cultured
bcl-2-Associated X Protein
biology
Estradiol
Chemistry
Reverse Transcriptase Polymerase Chain Reaction
Obstetrics and Gynecology
Polycystic ovary
APOPTOSIS
Nitric oxide synthase
Cytokine
medicine.anatomical_structure
Proto-Oncogene Proteins c-bcl-2
Female
POLYCYSTIC OVARY
Biología Reproductiva
CIENCIAS NATURALES Y EXACTAS
circulatory and respiratory physiology
medicine.drug
Polycystic Ovary Syndrome
endocrine system
medicine.medical_specialty
Blotting
Western
Ovary
Nitric Oxide
Real-Time Polymerase Chain Reaction
PROTAGLANDIN E2
Dinoprostone
Ciencias Biológicas
ANDROGENS
Bcl-2-associated X protein
Contraceptive Agents
Internal medicine
medicine
Animals
Androstenedione
RNA
Messenger
purl.org/becyt/ford/1.6 [https]
Cell Proliferation
Macrophages
Cell Biology
Rats
Disease Models
Animal
Reproductive Medicine
biology.protein
Hormone
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: Polycystic ovarian syndrome is the most common endocrine disorder among women of reproductive age. Little is known about its etiology, although the evidence suggests an intrinsic ovarian abnormality in which endocrine, metabolic, neural and immune factors would be involved. In this work, the effects of macrophage (MO) secretion on ovarian apoptosis in a polycystic ovary syndrome rat model (PCO rat) induced by estradiol valerate are studied. Spleen MO secretions were used to stimulate ovaries and ovarian interstitial and granulosa cells from both PCO and control rats. Ovarian hormones and prostaglandin E2 (PGE2) were measured by RIA; ovarian mRNA levels of Bax, Bcl2 and NFkB by RT-PCR; and ovarian inducible nitric oxide synthase (iNOS) by western blot. The number of apoptotic cells was evaluated by TUNEL. In the PCO ovary, the MO secretions from PCO rats increased the Bax and NFkB mRNA expressions and increased TUNEL staining in both granulosa and theca cells. In addition, the PCO MO secretions produced a decrease of nitric oxide release, iNOS protein level and PGE2 content in the PCO ovary, and it also induced an increase of androstenedione production by PCO interstitial cells, in comparison with control MO secretions. Considering these results and knowing that testosterone stimulates tumour necrosis factor-α production by PCO MO modifying ovarian response by increasing androstenedione, it is reasonable to suggest that the increase of androgens stimulated in ovarian cells by PCO MO secretions could in turn stimulate the cytokine production from MO, thus maintaining an apoptotic vicious cycle in the PCO ovary. Fil: Figueroa, Maria Florencia. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina Fil: Motta, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina Fil: Acosta, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina Fil: Oliveros, Liliana Beatriz. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina Fil: Forneris, Myriam Liliana. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Databáze: OpenAIRE
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