Molecular and clinical characterization of human adenovirus associated with acute respiratory tract infection in hospitalized children

Autor: Guixia Li, Meng-chuan Zhao, Ying-hui Guo, Shuo Yang, Fang-zhou Qiu, Le Wang, Zhishan Feng
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Acute respiratory tract infection
HBoV
Human bocavirus
Adenovirus Infections
Human
FluB
Influenza B
HCoV
Human coronavirus
0302 clinical medicine
Clinical characterization
Epidemiology
Genotype
Prevalence
Adenovirus
Longitudinal Studies
030212 general & internal medicine
Child
HMPV
Human metapneumovirus
Respiratory Tract Infections
Children
Phylogeny
Coinfection
ARTI
acute respiratory tract infection
virus diseases
CAP
community-acquired pneumonia
Hospitalization
Infectious Diseases
Child
Preschool
Molecular epidemiology
Acute Disease
RSV
Respiratory syncytial virus
Female
China
medicine.medical_specialty
Adolescent
09H1
Influenza A H1N1 pdm09
030106 microbiology
Mp
Mycoplasma pneumoniae
HRV
Rhinovirus
Article
03 medical and health sciences
HAdV
Human adenovirus
FluA
Influenza A
Virology
Internal medicine
medicine
Humans
Typing
HPIV
Human parainfluenza virus
Retrospective Studies
Ch
Chlamydia
H3
Influenza H3N2
business.industry
Adenoviruses
Human
Infant
Retrospective cohort study
Sequence Analysis
DNA
eye diseases
Hypervariable region
NPA
nasopharyngeal aspirate
DNA
Viral
business
Nested polymerase chain reaction
Zdroj: Journal of Clinical Virology
ISSN: 1386-6532
DOI: 10.1016/j.jcv.2019.104254
Popis: Highlights • 3.71% of children with ARTI exhibited HAdV positive. • HAdV-2, HAdV-3 and HAdV-7 were the predominant types identified from ARTI children. • 74.85% of HAdV were co-detected with other respiratory pathogens, most commonly HRV. • The co-detection rate of HAdV-C was significant higher than those of HAdV-B. • HAdV-7 positive children may not present more severe clinical outcome.
Background Human adenovirus (HAdV) is a common pathogen in children that can cause acute respiratory tract infection (ARTI), but the molecular epidemiological and clinical information relating to HAdV among hospitalized children with ARTI are few reported in China. Objectives To evaluate the epidemiological, clinical, and molecular characteristics of HAdV infections among hospitalized children with ARTI in Hebei, Northern China from June 2017 to May 2018. Study design A 12-month longitudinal, retrospective study on HAdV, typed by nested polymerase chain reaction targeting the hexon gene’s hypervariable region (typing was merely performed by sequencing of the hexon neutralization epitope and thus genotypes could not be identified unequivocally), associated with ARTI was performed. The epidemiological and clinical data of different types of HAdV were analyzed using statistical product and service solutions (SPSS) 21.0 software. Results HAdV was detected in 330 (3.71%) of the 8906 specimens, with most (88.48%, 292/330) HAdV-positives cases detected among children < 3 years old. HAdV were detected throughout the year with a higher prevalence in spring. 11 types were identified, with HAdV-2 (33.33%, 110/330) as the predominant type, followed by HAdV-3 (21.21%, 70/330) and HAdV-7 (13.94%, 46/330). Of the 330 HAdV-positive specimens, 247 (74.85%) were co-detected with other respiratory pathogens, most commonly rhinovirus (HRV) (58.7%, 145/247). Additionally, patients with HAdV-7 positive had longer duration of fever than HAdV-2 or -3 positive patients. Conclusions During the study period, HAdV-2, HAdV-3 and HAdV-7 were the predominant types identified from children with ARTI in Hebei Province. Pediatric patients with HAdV-7 positive may not present more severe clinical outcome except a longer duration of fever.
Databáze: OpenAIRE
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