Phylogenetic and epidemiological analysis of Neisseria meningitidis using DNA probes
| Autor: | K. A. V. Cartwright, A. I. Knight, Johnjoe McFadden, H. Ni |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Epidemiology
Biology Neisseria meningitidis Meningococcal disease medicine.disease_cause Disease Outbreaks Phylogenetics medicine Humans Typing Insertion sequence Serotyping Phylogeny Genetics Phylogenetic tree medicine.disease United Kingdom Meningococcal Infections Blotting Southern Infectious Diseases Genetic distance Evaluation Studies as Topic Restriction fragment length polymorphism DNA Probes Polymorphism Restriction Fragment Length Research Article |
| Zdroj: | Epidemiology and infection. 109(2) |
| ISSN: | 0950-2688 |
| Popis: | SUMMARYThe genetic relationships between various serotypes and serogroups of meningococcal strains were investigated by restriction fragment-length polymorphism (RFLP) analysis using a number of random DNA probes and a probe containing a truncated copy of the meningococcal insertion sequence IS1106. The data were used to estimate genetic distance between all pairs of strains and to construct phylogenetic trees for meningococcal strains. B15: P1.16R strains isolated from cases of systemic meningococcal disease in two health districts with a high incidence of disease were clonal in contrast to similar strains from cases occurring in other parts of the UK. Strains from these areas, which contain a similar genomic deletion, were found to be derived from two distinct lineages within the B15: P1.16R phylogenetic group. RFLP data demonstrated that present serological typing systems for the meningoccus do not necessarily reflect true genetic relationships. |
| Databáze: | OpenAIRE |
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