Seminal plasma reduces exogenous oxidative damage to human sperm, determined by the measurement of DNA strand breaks and lipid peroxidation
Autor: | R.J Potts, T.M Jefferies, L.J Notarianni |
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Rok vydání: | 2001 |
Předmět: |
Male
DNA damage Health Toxicology and Mutagenesis Semen DNA Fragmentation Biology medicine.disease_cause Thiobarbituric Acid Reactive Substances Lipid peroxidation chemistry.chemical_compound DNA Nucleotidylexotransferase Genetics medicine TBARS Humans Molecular Biology chemistry.chemical_classification Reactive oxygen species DNA Sperm Spermatozoa Oxidative Stress chemistry Biochemistry DNA fragmentation Lipid Peroxidation Oxidative stress DNA Damage |
Zdroj: | Mutation research. 447(2) |
ISSN: | 0027-5107 |
Popis: | Exposure of spermatozoa to reactive oxygen species (ROS) has been associated with cellular injury, that includes DNA damage and lipid peroxidation. In addition, sperm preparation techniques such as centrifugation, commonly used prior to in vitro fertilization and scientific studies, are associated with the generation of ROS and an increase in the level of DNA damage. The preservation, therefore, of sperm in vitro that might decrease the potential for oxidative DNA damage to arise and allow for an improvement in semen quality used for artificial insemination, is of importance. Seminal plasma is a rich source of antioxidants, which, potentially, safeguards sperm from oxidative attack during storage and once ejaculated. We have investigated the protection of human spermatozoa from ROS afforded by seminal plasma. Sperm were exposed to exogenous ROS by incubating the cells with hydrogen peroxide in the presence of ferrous sulfate and ADP. Aliquots of seminal plasma were added to the incubation mixture in differing amounts, and the generation of DNA strand breaks and thiobarbituric acid reactive species (TBARS), indicative of lipid peroxidation, determined. Incubation of sperm with exogenous ROS resulted in a significant generation of DNA strand breaks and lipid peroxidation compared to basal levels of damage (P |
Databáze: | OpenAIRE |
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