In situ hybridization and immunocytochemistry of alpha1-adrenoceptors in human peripheral blood lymphocytes
| Autor: | Alberto Ricci, Domenica Schiavone, Franco Veglio, Francesco Amenta, Marina Schena, Paolo Mulatero, Elena Bronzetti, Seyed Khosrow Tayebati, I. Rossodivita, S. Morra di Cella |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Lymphocyte Immunocytochemistry In situ hybridization In Vitro Techniques Transfection Receptors Adrenergic alpha-1 medicine Animals Humans Lymphocytes RNA Messenger Receptor In Situ Hybridization Pharmacology Messenger RNA biology General Neuroscience Molecular biology Immunohistochemistry Recombinant Proteins medicine.anatomical_structure COS Cells biology.protein Cytochemistry Antibody Immunostaining |
| Zdroj: | Journal of autonomic pharmacology. 20(5-6) |
| ISSN: | 0144-1795 |
| Popis: | 1 alpha1-Adrenoceptor subtypes were investigated in cytospin centrifuged preparations of human peripheral blood lymphocytes by in situ hybridization and immunocytochemistry. 2 In situ hybridization cytochemistry revealed alpha1A-, alpha1B-, and alpha1D-receptor mRNA in human peripheral blood lymphocytes. Lymphocytes hybridized for alpha1A receptor subtype represented approximately 30% of total lymphocytes, those hybridized for alpha1Beta- and alpha1D-receptor subtypes averaged 42 and 25% of total lymphocytes, respectively. 3 Cytospin centrifuged lymphocytes exposed to anti-alpha1A-, alpha1Beta- or alpha1D-receptor protein antibodies, developed specific immunostaining. Approximately 27% of total lymphocytes were immunoreactive for alpha1A-receptor protein, 40% displayed alpha1B-receptor protein immunoreactivity and 22% alpha1D-receptor protein immunoreactivity. Analysis of percentages as well as of lymphocyte morphology of in situ hybridized and immunolabelled lymphocytes suggests the co-expression of mRNA receptor signal and protein receptor immunostaining in the same lymphocyte. 4 The demonstration of both alpha1-adrenoceptor mRNA and receptor protein subtypes suggests that alpha1-adrenoceptors may have a role in regulating lymphocyte function. 5 The possibility of demonstrating receptor protein immunoreactivity in a small amount of blood, such as that required for preparing cytospin-centrifuged lymphocytes, may stimulate research to evaluate the role of these receptors in lymphocytes and to establish if assessment of lymphocyte alpha1-adrenoceptors may represent a marker of their status in health and disease. |
| Databáze: | OpenAIRE |
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