Rapid neutrophil accumulation and protein oxidation in irradiated rat lungs
| Autor: | M. Menard, Henry Fliss |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Time Factors Antioxidant Hypochlorous acid Neutrophils Physiology medicine.medical_treatment Glutathione reductase Biology Protein oxidation medicine.disease_cause Rats Sprague-Dawley Leukocyte Count chemistry.chemical_compound Methionine Cell Movement Physiology (medical) medicine Animals Sulfhydryl Compounds Lung Proteins Glutathione Molecular biology Blood Cell Count Hypochlorous Acid Rats medicine.anatomical_structure Biochemistry chemistry Tetradecanoylphorbol Acetate Oxidoreductases Oxidation-Reduction Oxidative stress |
| Zdroj: | Journal of Applied Physiology. 77:2727-2733 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/jappl.1994.77.6.2727 |
| Popis: | Exposure of lungs to high doses of ionizing radiation can initiate an injurious acute inflammatory response. We show that neutrophil content in the lungs of rats exposed to 10 Gy whole body gamma radiation increased threefold 4.5 h after irradiation and returned to normal by 24 h. Oxidized methionine in the proteins of the lungs, heart, liver, kidney, and jejunum increased significantly in 2 h. Treatment with the antioxidant dithiothreitol immediately after irradiation prevented methionine oxidation. Methionine oxidation was also observed after intrabronchial instillation of phorbol myristate acetate, a model of neutrophil oxidant-mediated pulmonary injury, as well as in isolated lungs perfused with hypochlorous acid, confirming the ability of neutrophil oxidants to cause protein oxidation in lungs. No change in glutathione or protein sulfhydryl content was detected in irradiated lungs 4.5 h after irradiation, possibly as a result of protection by the observed increase in pulmonary glutathione reductase. We therefore show that the acute pulmonary inflammatory response to radiation involves rapid neutrophil accumulation, oxidant production, and protein oxidation. |
| Databáze: | OpenAIRE |
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