Expression of caveolin-1 in tooth germ, ameloblastoma and ameloblastic carcinoma
| Autor: | Estefanía Sicco, Mariana Villarroel-Dorrego, Rogelio González-González, Celeste Sánchez-Romero, Ramón Gil Carreón-Burciaga, Gabriel Tapia-Repetto, Vanesa Pereira-Prado, Nelly Molina-Frechero, Marco Meleti, Mariana Suarez, Ronell Bologna-Molina |
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| Přispěvatelé: | Sánchez Romero Celeste, PhD. Molecular Pathology Area, Faculty of Dentistry, University of the Republic, Uruguay, Pereira Prado Vanesa, MSc. Molecular Pathology Area, Faculty of Dentistry, University of the Republic, Uruguay, Sicco Estefanía, MSc. Molecular Pathology Area, Faculty of Dentistry, University of the Republic, Uruguay, Suarez Mariana, MSc. Molecular Pathology Area, Faculty of Dentistry, University of the Republic, Uruguay, Tapia Repetto Gabriel, MSc. Molecular Pathology Area, Faculty of Dentistry, University of the Republic, Uruguay, Carreón Burciaga Ramón Gil, PhD. Oral Pathology, school of Dentistry, Universidad Juarez del Estado de Durango, Mexico, González González Rogelio, PhD. Oral Pathology, school of Dentistry, Universidad Juarez del Estado de Durango, Mexico, Villarroel Dorrego Mariana, PhD. Oral Histopathology Laboratory, Universidad Central de Venezuela, Venezuela, Meleti Marco, PhD. Department of Medicine and Surgery, Oral Medicine and Laser Surgery Unit, Centro Universitario di Odontoiatria, Uni versity of Parma, Italy, Molina Frechero Nelly, PhD. Health Care Department, Universidad Autónoma Metropolitana, Xochimilco, Mexico, Bologna Molina Ronell, PhD. Molecular Pathology Area, Faculty of Detistry, University of the Republic, Uruguay |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Real Time Polymerase Chain Reaction recurrence government.form_of_government Caveolin 1 Odontogenic Tumors Biology medicine.disease_cause Ameloblastic Carcinoma Malignant transformation Ameloblastoma Western blot Caveolin-1 medicine Humans Caveolina 1 resection General Dentistry curettage UNESCO:CIENCIAS MÉDICAS desmoplastic ameloblastoma Oral Medicine and Pathology medicine.diagnostic_test Research mixed radiopaque ? radiolucent Carcinoma Odontogenic tumor Tooth Germ medicine.disease Immunohistochemistry Ameloblastic carcinoma Otorhinolaryngology government Surgery Carcinogenesis enucleation |
| Zdroj: | Medicina Oral, Patología Oral y Cirugía Bucal Sánchez-Romero, C., Pereira-Prado, V., Sicco, E., Suarez, M., Tapia-Repetto, G., Carreón-Burciaga, R., Gónzalez-Gónzalez, R., Villarroel-Dorrego, M., Meleti, M., Molina-Frechero, N., & Bologna-Molina, N. (2021). Expression of caveolin-1 in tooth germ, ameloblastoma and ameloblastic carcinoma. En Medicina Oral Patología Oral y Cirugia Bucal (pp. e238-e245). Medicina Oral, S.L. https://doi.org/10.4317/medoral.24151 COLIBRI Universidad de la República instacron:Universidad de la República |
| ISSN: | 1698-6946 1698-4447 |
| DOI: | 10.4317/medoral.24151 |
| Popis: | Background The caveolin-1 protein (structural component of membrane caveolae) plays important roles in several biological functions, such as endocytosis, cell adhesion, and cell signaling. However, this protein has been associated with mechanisms of tumorigenesis in several neoplasms. The expression patterns and roles of caveolin-1 in the oral epithelium and in embryonic and odontogenic tumor tissues are still unclear. Material and Methods The expression of caveolin-1 was evaluated in samples of the normal gingival epithelium (n=7), human tooth germ (TG) (n=12), ameloblastoma (AM) (n=83), and ameloblastic carcinoma (AC) (n=9) by immunohistochemistry. Additionally, AM samples were analyzed by qRT-PCR and Western blot. Results Most TG (91.7%), AM (73.5%) and AC (100%) samples showed diverse patterns of immunohistochemical positivity for caveolin-1, while only one gingival sample was positive. The transcript levels of cav-1 were significantly upregulated by 14.9-fold in AM tissue (P = 0.0014) compared to those in normal gingival epithelial tissue, as shown by qRT-PCR. Presence of caveolin-1 protein was confirmed by Western blot analysis. The caveolin-1 immunoexpression patterns throughout the stages of TG show its importance during odontogenesis. Conclusions The overexpression of caveolin-1 in AM and AC compared to its expression in normal gingival epithelium (adult tissue) suggests a possible role of caveolin-1 in protumoral events, but due to the similar immunoexpression observed in AM and AC, caveolin-1 may not necessarily participate in the malignant transformation process. However, future studies are needed to clarify and confirm these hypotheses. Key words:Ameloblastoma, ameloblastic carcinoma, caveolin-1, immunohistochemistry, real-time polymerase chain reaction. |
| Databáze: | OpenAIRE |
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